Urine and Dried Blood Spots From Children and Pregnant Women Reveal Phytochemicals, Amino Acids, and Carnitine Metabolites as Cowpea Consumption Biomarkers.

cowpea dietary exposure biomarkers dried dlood spots metabolism urine

Journal

Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818

Informations de publication

Date de publication:
17 Jan 2024
Historique:
revised: 23 10 2023
received: 11 04 2023
medline: 18 1 2024
pubmed: 18 1 2024
entrez: 17 1 2024
Statut: aheadofprint

Résumé

Legumes consumption has been proven to promote health across the lifespan; cowpeas have demonstrated efficacy in combating childhood malnutrition and growth faltering, with an estimated malnutrition prevalence of 35.6% of children in Ghana. This cowpea feeding study aimed to identify a suite of metabolic consumption biomarkers in children and adults. Urine and dried blood spots (DBS) from 24 children (9-21 months) and 21 pregnant women (>18 years) in Northern Ghana are collected before and after dose-escalated consumption of four cowpea varieties for 15 days. Untargeted metabolomics identified significant increases in amino acids, phytochemicals, and lipids. The carnitine metabolism pathway is represented by 137 urine and 43 DBS metabolites, with significant changes to tiglylcarnitine and acetylcarnitine. Additional noteworthy candidate biomarkers are mansouramycin C, N-acetylalliin, proline betaine, N2, N5-diacetylornithine, S-methylcysteine, S-methylcysteine sulfoxide, and cis-urocanate. S-methylcysteine and S-methylcysteine sulfoxide are targeted and quantified in urine. This feeding study for cowpea biomarkers supports the utility of a suite of key metabolites classified as amino acids, lipids, and phytochemicals for dietary legume and cowpea-specific food exposures of global health importance.

Identifiants

pubmed: 38233141
doi: 10.1002/mnfr.202300222
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2300222

Subventions

Organisme : United States Agency for International Development
ID : 7200AA18LE00003

Informations de copyright

© 2024 Wiley-VCH GmbH.

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Auteurs

Madison Tipton (M)

Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, 80523, USA.

Bridget A Baxter (BA)

Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, 80523, USA.

Brigitte A Pfluger (BA)

Nutrition and Health Sciences, Laney Graduate School, Emory University, Atlanta, Georgia, 30322, USA.

Brooke Sayre-Chavez (B)

Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado, 80521, USA.

María Muñoz-Amatriaín (M)

Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado, 80521, USA.
Departamento de Biología Molecular - Área de Genética, Universidad de León, León, 24071, Spain.

Corey D Broeckling (CD)

Analytical Resources Core: Bioanalysis and Omics Center, Colorado State University, Fort Collins, Colorado, 80523, USA.

Issah Shani (I)

Department of Nutrition and Food Science, College of Basic and Applied Science, University of Ghana, Legon, Accra, P.O. Box LG 134 Legon, Ghana.

Matilda Steiner-Asiedu (M)

Department of Nutrition and Food Science, College of Basic and Applied Science, University of Ghana, Legon, Accra, P.O. Box LG 134 Legon, Ghana.

Mark Manary (M)

Department of Pediatrics, Washington University School of Medicine in St. Louis, St. Louis, Missouri, 63110, USA.

Elizabeth P Ryan (EP)

Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, 80523, USA.

Classifications MeSH