Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platforms.
BY-2 cell culture
COVID-19 pandemic
RBD production
diagnostics
glycan profiles
plant-based biologics
serological tests
transient expression
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2023
2023
Historique:
received:
20
10
2023
accepted:
04
12
2023
medline:
19
1
2024
pubmed:
19
1
2024
entrez:
19
1
2024
Statut:
epublish
Résumé
The COVID-19 pandemic has underscored the need for rapid and cost-effective diagnostic tools. Serological tests, particularly those measuring antibodies targeting the receptor-binding domain (RBD) of the virus, play a pivotal role in tracking infection dynamics and vaccine effectiveness. In this study, we aimed to develop a simple enzyme-linked immunosorbent assay (ELISA) for measuring RBD-specific antibodies, comparing two plant-based platforms for diagnostic reagent production. We chose to retain RBD in the endoplasmic reticulum (ER) to prevent potential immunoreactivity issues associated with plant-specific glycans. We produced ER-retained RBD in two plant systems: a stable transformation of BY-2 plant cell culture (BY2-RBD) and a transient transformation in
Identifiants
pubmed: 38239207
doi: 10.3389/fpls.2023.1325162
pmc: PMC10794598
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1325162Informations de copyright
Copyright © 2024 Santoni, Gutierrez-Valdes, Pivotto, Zanichelli, Rosa, Sobrino-Mengual, Balieu, Lerouge, Bardor, Cecchetto, Compri, Mazzariol, Ritala and Avesani.
Déclaration de conflit d'intérêts
Authors MS and ARo were employed by company Diamante SB srl. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.