Identification of a Tetrahymena species infecting guppies, pathology, and expression of beta-tubulin during infection.
Guppy
Infection
Tetrahymena pyriformis
Tetrahymenosis
β-Tubulin
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
19 Jan 2024
19 Jan 2024
Historique:
received:
04
09
2023
accepted:
04
01
2024
medline:
19
1
2024
pubmed:
19
1
2024
entrez:
19
1
2024
Statut:
epublish
Résumé
Tetrahymenosis is caused by the ciliated protozoan Tetrahymena and is responsible for serious economic losses to the aquaculture industry worldwide. However, information regarding the molecular mechanism leading to tetrahymenosis is limited. In previous transcriptome sequencing work, it was found that one of the two β-tubulin genes in T. pyriformis was significantly expressed in infected fish, we speculated that β-tubulin is involved in T. pyriformis infecting fish. Herein, the potential biological function of the β-tubulin gene in Tetrahymena species when establishing infection in guppies was investigated by cloning the full-length cDNA of this T. pyriformis β-tubulin (BTU1) gene. The full-length cDNA of T. pyriformis BTU1 gene was 1873 bp, and the ORF occupied 1134 bp, whereas 5' UTR 434 bp, and 3' UTR 305 bp whose poly (A) tail contained 12 bases. The predicted protein encoded by T. pyriformis BTU1 gene had a calculated molecular weight of 42.26 kDa and pI of 4.48. Moreover, secondary structure analysis and tertiary structure prediction of BTU1 protein were also conducted. In addition, morphology, infraciliature, phylogeny, and histopathology of T. pyriformis isolated from guppies from a fish market in Harbin were also investigated. Furthermore, qRT-PCR analysis and experimental infection assays indicated that the expression of BTU1 gene resulted in efficient cell proliferation during infection. Collectively, our data revealed that BTU1 is a key gene involved in T. pyriformis infection in guppies, and the findings discussed herein provide valuable insights for future studies on tetrahymenosis.
Identifiants
pubmed: 38240890
doi: 10.1007/s00436-024-08117-0
pii: 10.1007/s00436-024-08117-0
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104Subventions
Organisme : the Natural Science Foundation of China
ID : 31970498
Organisme : the Natural Science Foundation of China
ID : 31970498
Organisme : the Natural Science Foundation of China
ID : 31970498
Organisme : the Natural Science Foundation of China
ID : 31970498
Organisme : the Natural Science Foundation of China
ID : 31970498
Organisme : the Natural Science Foundation of China
ID : 31970498
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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