Protocol for measuring mitochondrial size in mouse and human liver tissues.
Cell Biology
Health Sciences
Metabolism
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
15 Mar 2024
15 Mar 2024
Historique:
received:
23
10
2023
revised:
10
12
2023
accepted:
05
01
2024
pubmed:
20
1
2024
medline:
20
1
2024
entrez:
20
1
2024
Statut:
ppublish
Résumé
Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol for measuring mitochondrial size through immunofluorescence staining, confocal imaging, and analysis in ImageJ. We describe the steps for tissue processing, antigen retrieval, mitochondrial staining using an integrating immunofluorescence assay, and computerized image analysis to measure each mitochondrial size in mouse and human liver tissues. This protocol reduces tissue sample volume and processing time for the preparation of primary cells. For complete details on the use and execution of this protocol, please refer to Pearah et al.
Identifiants
pubmed: 38244201
pii: S2666-1667(24)00007-8
doi: 10.1016/j.xpro.2024.102842
pmc: PMC10831311
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102842Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK107641
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK120309
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK129919
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM144103
Pays : United States
Informations de copyright
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.