A Modular Microfluidic Organoid Platform Using LEGO

The convergence of organoid and organ-on-a-chip (OoC) technologies is urgently needed to overcome limitations of current 3D in vitro models. However, integrating organoids in standard OoCs faces several technical challenges as it is typically laborious, lacks flexibility and often results in even more complex and less efficient cell culture protocols. Therefore, specifically adapted and more flexible microfluidic platforms need to be developed to facilitate the incorporation of complex 3D in vitro models. Here, we developed a modular, tubeless fluidic circuit board (FCB) coupled with reversibly sealed cell culture bricks for dynamic culture of embryonic stem cell (ESC)-derived thyroid follicles. The FCB was fabricated by milling channels in a polycarbonate (PC) plate followed by thermal bonding against another PC plate. LEGO

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