Diversity and ecological function of urease-producing bacteria in the cultivation environment of Gracilariopsis lemaneiformis.
Diversity
Ecological function
Gracilariopsis lemaneiformis
Urease
Urease-producing bacteria (UPB)
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
23 Jan 2024
23 Jan 2024
Historique:
received:
10
08
2023
accepted:
27
12
2023
medline:
23
1
2024
pubmed:
23
1
2024
entrez:
23
1
2024
Statut:
epublish
Résumé
Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by hydrolyzing urea, and play an important role in marine nitrogen cycle. However, there is still an incomplete understanding of UPB and their ecological functions in the cultivation environment of the red macroalgae Gracilariopsis lemaneiformis. This study comprehensively analyzed the diversity of culturable UPB and explored their effects on urea uptake by G. lemaneiformis. A total of 34 isolates belonging to four main bacterial phyla i.e. (Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria) were identified through 16S rRNA sequencing and were screened for UPB by urea agar chromogenic medium assay and ureC gene cloning. Our data revealed that only 8 strains contained urease. All of these UPB exhibited different urease activities, which were determined by the Berthelot reaction colorimetry assay. Additionally, the UPB strain (G13) isolated from G. lemaneiformis with higher urease activity was selected for co-culture with G. lemaneiformis to explore its role in promoting or inhibiting nitrogen uptake by macroalgae. The results showed a significant increase in urea consumption in the culture medium and the total cellular nitrogen in G. lemaneiformis in the UPB-co culture group compared to the sterile group. This suggests that the selected UPB strain positively influences nitrogen uptake by G. lemaneiformis. Similarly, isotopic assays revealed that the δ
Identifiants
pubmed: 38261068
doi: 10.1007/s00248-023-02339-y
pii: 10.1007/s00248-023-02339-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
35Subventions
Organisme : the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province
ID : 2022fjscq02
Organisme : the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province
ID : 2022fjscq02
Organisme : the National Natural Science Foundation of China
ID : 41976125
Organisme : the Natural Science Foundation of China grants
ID : 42206116
Organisme : the Program for University Innovation Team of Guangdong Province
ID : 2022KCXTD008
Organisme : the Team Project of Department of Education of Guangdong Province
ID : 2018KCXTD012
Informations de copyright
© 2024. The Author(s).
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