The nucleolar shell provides anchoring sites for DNA untwisting.

Journal

Communications biology

ISSN: 2399-3642

Titre abrégé: Commun Biol

Pays: England

ID NLM: 101719179

Informations de publication

Date de publication:
23 Jan 2024

Historique:

received: 18 08 2023

accepted: 28 12 2023

medline: 24 1 2024

pubmed: 24 1 2024

entrez: 23 1 2024

Statut: epublish

Résumé

DNA underwinding (untwisting) is a crucial step in transcriptional activation. DNA underwinding occurs between the site where torque is generated by RNA polymerase (RNAP) and the site where the axial rotation of DNA is constrained. However, what constrains DNA axial rotation in the nucleus is yet unknown. Here, we show that the anchorage to the nuclear protein condensates constrains DNA axial rotation for DNA underwinding in the nucleolus. In situ super-resolution imaging of underwound DNA reveal that underwound DNA accumulates in the nucleolus, a nuclear condensate with a core-shell structure. Specifically, underwound DNA is distributed in the nucleolar core owing to RNA polymerase I (RNAPI) activities. Furthermore, underwound DNA in the core decreases when nucleolar shell components are prevented from binding to their recognition structure, G-quadruplex (G4). Taken together, these results suggest that the nucleolar shell provides anchoring sites that constrain DNA axial rotation for RNAPI-driven DNA underwinding in the core. Our findings will contribute to understanding how nuclear protein condensates make up constraints for the site-specific regulation of DNA underwinding and transcription.

Identifiants

DOI: 10.1038/s42003-023-05750-w PMID: 38263258

pubmed: 38263258

doi: 10.1038/s42003-023-05750-w

pii: 10.1038/s42003-023-05750-w

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

Subventions

Organisme : MEXT | JST | Core Research for Evolutional Science and Technology (CREST)

ID : JPMJCR2023

Organisme : MEXT | JST | Core Research for Evolutional Science and Technology (CREST)

ID : JPMJCR22L5

Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)

ID : JP20K20180

Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)

ID : JP23KJ1255

Organisme : MEXT | Japan Science and Technology Agency (JST)

ID : JPMJSP2110

Informations de copyright

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