Programmed disassembly of a microtubule-based membrane protrusion network coordinates 3D epithelial morphogenesis in Drosophila.
Cellular Protrusion
Epithelial Morphogenesis
Microtubule Dynamics
Non-Centrosomal Microtubule Organizing Center
Three-Dimensional Morphogenesis
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
23 Jan 2024
23 Jan 2024
Historique:
received:
01
03
2023
accepted:
18
12
2023
revised:
14
12
2023
medline:
24
1
2024
pubmed:
24
1
2024
entrez:
23
1
2024
Statut:
aheadofprint
Résumé
Comprehensive analysis of cellular dynamics during the process of morphogenesis is fundamental to understanding the principles of animal development. Despite recent advancements in light microscopy, how successive cell shape changes lead to complex three-dimensional tissue morphogenesis is still largely unresolved. Using in vivo live imaging of Drosophila wing development, we have studied unique cellular structures comprising a microtubule-based membrane protrusion network. This network, which we name here the Interplanar Amida Network (IPAN), links the two wing epithelium leaflets. Initially, the IPAN sustains cell-cell contacts between the two layers of the wing epithelium through basal protrusions. Subsequent disassembly of the IPAN involves loss of these contacts, with concomitant degeneration of aligned microtubules. These processes are both autonomously and non-autonomously required for mitosis, leading to coordinated tissue proliferation between two wing epithelia. Our findings further reveal that a microtubule organization switch from non-centrosomal to centrosomal microtubule-organizing centers (MTOCs) at the G2/M transition leads to disassembly of non-centrosomal microtubule-derived IPAN protrusions. These findings exemplify how cell shape change-mediated loss of inter-tissue contacts results in 3D tissue morphogenesis.
Identifiants
pubmed: 38263333
doi: 10.1038/s44318-023-00025-w
pii: 10.1038/s44318-023-00025-w
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Academy of Finland (AKA)
ID : 347569
Organisme : Academy of Finland (AKA)
ID : 272280
Organisme : Eesti Teadusagentuur (ETAg)
ID : MOBERC33
Informations de copyright
© 2024. The Author(s).
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