Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: A snapshot study.
EPISEQ® 16S
Proteus sp
anaerobic bacteria
metagenomics next-generation sequencing
pressure ulcer
spinal cord injury
urinary microbiota
wound evolution
Journal
International wound journal
ISSN: 1742-481X
Titre abrégé: Int Wound J
Pays: England
ID NLM: 101230907
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
received:
05
10
2023
accepted:
14
12
2023
medline:
26
1
2024
pubmed:
26
1
2024
entrez:
25
1
2024
Statut:
ppublish
Résumé
Current microbiome investigations of patients with pressure ulcers (PU) are mainly based on wound swabs and/or biopsy sequencing, leaving the colonization scenario unclear. Urinary microbiota has been never studied. As a part of the prospective ESCAFLOR study, we studied urinary microbiota of spinal cord injury (SCI) patients with PU without any urinary tract infection at the inclusion, collected at two times (at admission [D0] and after 28 days [D28]) during the patient's care, investigated by 16S rDNA metagenomics next generation sequencing. Subgroup analyses were carried out between patients with wounds showing improved evolution versus stagnated/worsened wounds at D28. Analysis was done using EPISEQ® 16S and R software. Among the 12 studied patients, the urinary microbiota of patients with improved wound evolution at D28 (n = 6) presented a significant decrease of microbial diversity. This modification was associated with the presence of Proteobacteria phylum and an increase of Escherichia-Shigella (p = 0.005), as well as the presence of probiotic anaerobic bacteria Lactobacillus and Bifidobacterium. In contrast, Proteus abundance was significantly increased in urine of patients with stagnated/worsened wound evolution (n = 6) (p = 0.003). This study proposes urinary microbiota as a complementary factor indirectly associated with the wound evolution and patient cure. It opens new perspectives for further investigations based on multiple body microbiome comparison to describe the complete scenario of the transmission dynamics of wound-colonizing microorganisms.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14626Subventions
Organisme : Centre Hospitalier Universitaire de Nîmes
Organisme : French Society of Pressure Ulcer
Informations de copyright
© 2024 The Authors. International Wound Journal published by Medicalhelplines.com Inc and John Wiley & Sons Ltd.
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