Activation of murine macrophages by membrane proteins from Tritrichomonas foetus grown on iron- and calcium-rich conditions.
Tritrichomonas foetus
calcium
immunomodulatory
iron
macrophages
Journal
Parasite immunology
ISSN: 1365-3024
Titre abrégé: Parasite Immunol
Pays: England
ID NLM: 7910948
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
revised:
08
12
2023
received:
17
07
2023
accepted:
11
12
2023
medline:
26
1
2024
pubmed:
26
1
2024
entrez:
26
1
2024
Statut:
ppublish
Résumé
Tritrichomonas foetus is a protozoan parasite that causes a venereal disease in cattle limiting reproduction by abortions and sterility. The immune response against this parasite is poorly understood. Since the iron and calcium ions are important regulators of the microenvironment of the urogenital tract in cattle, we decided to evaluate the role of these divalent cations on the antigenicity of membrane proteins of T. foetus on macrophage activation as one of the first inflammatory responses towards this pathogen. Colorimetric methods and ELISA were used to detect the nitric oxide and oxygen peroxide production and expression of cytokines in culture supernatant from macrophage incubated with membrane proteins from T. foetus cultured in iron- and calcium-rich conditions. qRT-PCR assays were used to evaluate the transcript expression of genes involved in the inflammatory response on the macrophages. The membrane proteins used for in vitro stimulation caused the up-regulation of the iNOS and NOX-2 genes as well as the generation of NO and H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13020Informations de copyright
© 2024 John Wiley & Sons Ltd.
Références
Doumecq ML, Soto P. Tritrichomonas foetus: mecanismos de acción patógena. InVet. 2012;14:151-161.
Tolbert MK, Gookin JL. Mechanisms of Tritrichomonas foetus pathogenicity in cats with insights from venereal trichomonosis. J Vet Int Med. 2016;30:516-526.
Campero CM, Cobo ER. Tritrichomonas foetus: pathogenesis of embryo/fetal mortality, antigenic vaccine characterization and immune induced response. Rev Med Vet (Buenos Aires). 2006;87:47-56.
Irons PC, McGowan M, de Assis PM, et al. Prevalence of Tritrichomonas foetus in beef bulls slaughtered at two abattoirs in northern Australia. Aust Vet J. 2022;100:201-204.
Kulda J, Cerkasov J, Demes P, Cerkasovova A. Tritrichomonas foetus: stable anaerobic resistance to metronidazole in vitro. Exp Parasitol. 1984;57:93-103.
Herr S, Ribeiro LM, Claassen E, Myburgh JG. A reduction in the duration of infection with Tritrichomonas foetus following vaccination in heifers and the failure to demonstrate a curative effect in infected bulls. Onderstepoort J Vet Res. 1991;58:41-45.
Ortega-Mora LM, Sánchez-Sánchez R, Rojo-Montejo S, et al. A new inactivated Tritrichomonas foetus vaccine that improves genital clearance of the infection and calving intervals in cattle. Front Vet Sci. 2022;9:1005556.
Mendoza-Oliveros T, Arana-Argáez V, Alvaréz-Sánchez LC, Lara-Riegos J, Alvaréz-Sánchez ME, Torres-Romero JC. Immune response of BALB/c mice toward putative calcium transporter recombinant protein of Trichomonas vaginalis. Korean J Parasitol. 2019;57:33-38.
Zhang Z, Li Y, Wang S, et al. The molecular characterization and immunity identification of Trichomonas vaginalis adhesion protein 33 (AP33). Front Microbiol. 2020;11:1433.
Lee HY, Hyung S, Lee JW, et al. Identification of antigenic proteins in Trichomonas vaginalis. Korean J Parasitol. 2011;49:79-83.
Vaňáčová Š, Rasoloson D, Rázga J, Hrdý I, Kulda J, Tachezy J. Iron-induced changes in pyruvate metabolism of Tritrichomonas foetus and involvement of iron in expression of hydrogenosomal proteins. Microbiology. 2001;147:53-62.
Melo-Braga MB, da Rocha-Azevedo B, Silva-Filho FC. Tritrichomonas foetus: the role played by iron during parasite interaction with epithelial cells. Exp Parasitol. 2003;105:111-120.
Petropolis DB, Rodrigues JCF, da Rocha-Azevedo B, Silva-Filho FC. The binding of Tritrichomonas foetus to immobilized laminin-1 and its role in the cytotoxicity exerted by the parasite. Microbiology. 2008;15:2283-2290.
Alavi-Shoushtari SM, Asri-Rezaie S, Abedizadeh R, Khaki A, Pak M, Alizadeh S. Calcium and magnesium concentrations in uterine fluid and blood serum during the estrous cycle in the bovine. Vet Res Forum. 2012;3:137-141.
Tvrdá E, Lukáč N, Schneidgenová M, et al. Impact of seminal chemical elements on the oxidative balance in bovine seminal plasma and spermatozoa. J Vet Med. 2013;2013:1-8.
Han IH, Goo SY, Park SJ, et al. Proinflammatory cytokine and nitric oxide production by human macrophages stimulated with Trichomonas vaginalis. Korean J Parasitol. 2009;47:205-212.
Li L, Li X, Gong P, et al. Trichomonas vaginalis induces production of proinflammatory cytokines in mouse macrophages through activation of MAPK and NF-κB pathways partially mediated by TLR2. Front Microbiol. 2018;9:712.
Anderson ML, BonDurant RH, Corbeil RR, Corbeil LB. Immune and inflammatory responses to reproductive tract infection with Tritrichomonas foetus in immunized and control heifers. J Parasitol. 1996;82(4):594-600.
Dąbrowska J, Karamon J, Kochanowski M, Sroka J, Zdybel J, Cencek T. Tritrichomonas foetus as a causative agent of Tritrichomonosis in different animal hosts. J Vet Res. 2019;63(4):533-541.
De Azevedo NL, Silva Filho FC, De Souza W. The interaction of Tritrichomonas foetus and Trichomonas vaginalis with macrophages. J Submicrosc Cytol Pathol. 1991;23(2):319-326.
Monteavaro CE, Aguirre JI, Soto P, et al. Interaction of Tritrichomonas foetus with the reproductive tract of experimentally infected female BALB/c mice: ultrastructural evaluation. Vet J (London, England: 1997). 2007;173(1):204-208.
Cobo ER, Cano D, Rossetti O, Campero CM. Heifers immunized with whole-cell and membrane vaccines against Tritrichomonas foetus and naturally challenged with an infected bull. Vet Parasitol. 2002;109(3-4):169-184.
Ceballos-Góngora E, Torres-Romero JC, Arana-Argáez VE, et al. Exposure of Tritrichomonas foetus to sublethal doses of metronidazole induces a specific proinflammatory response in murine macrophages. J Eukaryot Microbiol. 2023;e13000. doi:10.1111/jeu.13000.
Arana-Argáez VE, Ceballos-Góngora E, Alvarez-Sánchez ME, Euan-Canto A, Lara-Riegos J, Torres-Romero JC. In vitro activation of macrophages by an MHC class II-restricted Trichomonas vaginalis TvZIP8-derived synthetic peptide. Immunol Invest. 2022;51:88-102.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem. 1982;126:131-138.
Pick E, Mizel D. Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods. 1981;46:211-226.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001;25:402-408.
Vijay K. Toll-like receptors in immunity and inflammatory diseases: past, present, and future. Int Immunopharmacol. 2018;59:391-412.
Verbeke R, Hogan MJ, Loré K, Pardi N. Innate immune mechanisms of mRNA vaccines. Immunity. 2022;55(11):1993-2005.
Dadarwal D, Palmer C, Griebel P. Mucosal immunity of the postpartum bovine genital tract. Theriogenology. 2017;104:62-71.
Song MJ, Lee JJ, Nam YH, et al. Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products. BMB Rep. 2015;48:103-108.
Medina Sanchez L, Siller M, Zeng Y, et al. The gut protist Tritrichomonas arnold restrains virus-mediated loss of oral tolerance by modulating dietary antigen-presenting dendritic cells. Immunity. 2023;56(8):1862-1875.e9.
Rutkowski MR, McNamee LA, Harmsen AG. Neutrophils and inducible nitric-oxide synthase are critical for early resistance to the establishment of Tritrichomonas foetus infection. J Parasitol. 2007;93:562-574.
Rutkowski MR, Harmsen AG. Tritrichomonas foetus: pathogenesis of acute infection in normal, estradiol-treated, and stressed mice. Exp Parasitol. 2007;115:143-159.
Liu C, Chu D, Kalantar-Zadeh K, George J, Young HA, Liu G. Cytokines: from clinical significance to quantification. Adv Sci. 2021;8:2004433.
Mukherjee S, Karmakar S, Babu SPS. TLR2 and TLR4 mediated host immune responses in major infectious diseases: a review. Braz J Infect Dis. 2016;20:193-204.