Identification of differentially recognized T cell epitopes in the spectrum of tuberculosis infection.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 Jan 2024
26 Jan 2024
Historique:
received:
13
04
2023
accepted:
12
01
2024
medline:
27
1
2024
pubmed:
27
1
2024
entrez:
26
1
2024
Statut:
epublish
Résumé
There is still incomplete knowledge of which Mycobacterium tuberculosis (Mtb) antigens can trigger distinct T cell responses at different stages of infection. Here, a proteome-wide screen of 20,610 Mtb-derived peptides in 21 patients mid-treatment for active tuberculosis (ATB) reveals IFNγ-specific T cell responses against 137 unique epitopes. Of these, 16% are recognized by two or more participants and predominantly derived from cell wall and cell processes antigens. There is differential recognition of antigens, including TB vaccine candidate antigens, between ATB participants and interferon-gamma release assay (IGRA + /-) individuals. We developed an ATB-specific peptide pool (ATB116) consisting of epitopes exclusively recognized by ATB participants. This pool can distinguish patients with pulmonary ATB from IGRA + /- individuals from various geographical locations, with a sensitivity of over 60% and a specificity exceeding 80%. This proteome-wide screen of T cell reactivity identified infection stage-specific epitopes and antigens for potential use in diagnostics and measuring Mtb-specific immune responses.
Identifiants
pubmed: 38278794
doi: 10.1038/s41467-024-45058-9
pii: 10.1038/s41467-024-45058-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
765Subventions
Organisme : NIAID NIH HHS
ID : 75N93019C00067
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI137681
Pays : United States
Organisme : NIAID NIH HHS
ID : 75N93019C00067
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272200900044C
Pays : United States
Informations de copyright
© 2024. The Author(s).
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