Protective effect of Bifidobacterium animalis CGMCC25262 on HaCaT keratinocytes.
Bifidobacterium animalis
Cosmetic material
Keratinocyte HaCaT
Probiotic role
Skin barrier
Journal
International microbiology : the official journal of the Spanish Society for Microbiology
ISSN: 1618-1905
Titre abrégé: Int Microbiol
Pays: Switzerland
ID NLM: 9816585
Informations de publication
Date de publication:
27 Jan 2024
27 Jan 2024
Historique:
received:
08
11
2023
accepted:
19
01
2024
revised:
09
01
2024
medline:
27
1
2024
pubmed:
27
1
2024
entrez:
26
1
2024
Statut:
aheadofprint
Résumé
Bifidobacteria are the most prevalent members of the intestinal microbiota in mammals and other animals, and they play a significant role in promoting gut health through their probiotic effects. Recently, the potential applications of Bifidobacteria have been extended to skin health. However, the beneficial mechanism of Bifidobacteria on the skin barrier remains unclear. In this study, keratinocyte HaCaT cells were used as models to evaluate the protective effects of the cell-free supernatant (CFS), heat-inactivated bacteria, and bacterial lysate of Bifidobacterium animalis CGMCC25262 on the skin barrier and inflammatory cytokines. The results showed that all the tested samples were able to upregulate the transcription levels of biomarker genes associated with the skin barrier, such as hyaluronic acid synthetase (HAS) and aquaporins (AQPs). Notably, the transcription of the hyaluronic acid synthetase gene-2 (HAS-2) is upregulated by 3~4 times, and AQP3 increased by 2.5 times when the keratinocyte HaCaT cells were co-incubated with 0.8 to 1% CFS. In particular, the expression level of Filaggrin (FLG) in HaCaT cells increased by 1.7 to 2.7 times when incubated with Bifidobacterial samples, reaching its peak at a concentration of 0.8% CFS. Moreover, B. animalis CGMCC25262 also decreased the expression of the proinflammatory cytokine RANTES to one-tenth compared to the levels observed in HaCaT cells induced with tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ). These results demonstrate the potential of B. animalis CGMCC25262 in protecting the skin barrier and reducing inflammatory response.
Identifiants
pubmed: 38278974
doi: 10.1007/s10123-024-00485-y
pii: 10.1007/s10123-024-00485-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
Références
Abir-Awan M, Kitchen P et al (2019) Inhibitors of mammalian aquapor in water channels. Int J Mol Sci 20(7):22. https://doi.org/10.3390/ijms20071589
doi: 10.3390/ijms20071589
Amaretti A, di Nunzio M et al (2013) Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl Microbiol Biotechnol 97(2). https://doi.org/10.1007/s00253-012-4241-7
Andrew WB, Richard LG (2011) The coordinated response of the physical and antimicrobial peptide barriers of the skin. J Invest Dermatol 131(2). https://doi.org/10.1038/jid.2010.360
Arck P, Handjiski B et al (2010) Is there a 'gut-brain-skin axis'? Exp Dermatol 19(5). https://doi.org/10.1111/j.1600-0625.2009.01060.x
Bieber T (2022) Atopic dermatitis: an expanding therapeutic pipeline for a complex disease. Nat Rev Drug Discov 21(1):20. https://doi.org/10.1038/s41573-021-00266-6
doi: 10.1038/s41573-021-00266-6
Boada A, Bielsa I et al (2010) Perniosis: clinical and histopathological analysis. Am J Dermatopathol 32(1). https://doi.org/10.1097/DAD.0b013e3181af1d24
Boguniewicz M, Schmid-Grendelmeier P et al (2006) Atopic dermatitis. J Allergy Clin Immunol 118(1):4. https://doi.org/10.1016/j.jaci.2005.10.018
doi: 10.1016/j.jaci.2005.10.018
Cushing CA, Phillips LG (2013) Evidence-based medicine: pressure sores. Plast Reconstr Surg 132(6). https://doi.org/10.1097/PRS.0b013e3182a808ba
Dainichi T, Hanakawa S et al (2014) Classification of inflammatory skin diseases: a proposal based on the disorders of the three-layered defense systems, barrier, innate immunity and acquired immunity. J Dermatol Sci 76(2):81–89. https://doi.org/10.1016/j.jdermsci.2014.08.010
doi: 10.1016/j.jdermsci.2014.08.010
pubmed: 25242498
De Rodas BZ, Gilliland SE et al (1996) Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J Dairy Sci 79(12). https://doi.org/10.3390/foods11091283
Deguchi Y, Morishita T et al (1985) Comparative studies on synthesis of water-soluble vitamins among human species of Bifidobacteria. Agric Biol Chem 49(1):13–19. https://doi.org/10.1080/00021369.1985.10866683
doi: 10.1080/00021369.1985.10866683
Duarte M, Oliveira AL et al (2022) Current postbiotics in the cosmetic market-an update and development opportunities. Appl Microbiol Biotechnol 106(18):5879–5891. https://doi.org/10.1007/s00253-022-12116-5
doi: 10.1007/s00253-022-12116-5
pubmed: 36008565
Fijan S (2014) Microorganisms with claimed probiotic properties: an overview of recent literature. Int J Environ Res Public Health 11(5):4745–4767. https://doi.org/10.3390/ijerph110504745
doi: 10.3390/ijerph110504745
pubmed: 24859749
pmcid: 4053917
Gabriela O, Jesse CL et al (2014) The effects of heat on skin barrier function and in vivo dermal absorption. Int J Pharm 464(1-2). https://doi.org/10.1016/j.ijpharm.2014.01.012
Gao H, Yang B et al (2020) Characteristics of bifidobacterial conjugated fatty acid and hydroxy fatty acid production and its potential application in fermented milk. LWT- Food Sci Technol 120. https://doi.org/10.1016/j.lwt.2019.108940
Gewiss C, Augustin M (2023) Recent insights into comorbidities in atopic dermatitis. Expert Rev Clin Immunol 19(4):12. https://doi.org/10.1080/1744666X.2023.2181790
doi: 10.1080/1744666X.2023.2181790
Goderska K (2019) The antioxidant and prebiotic properties of lactobionic acid. Appl Microbiol Biotechnol 103(9). https://doi.org/10.1007/s00253-019-09754-7
Gomes C, Silva AC et al (2020) Biotechnology applied to cosmetics and aesthetic medicines. Cosmetics 7(2). https://doi.org/10.3390/cosmetics7020033
Gueniche A, Bastien P et al (2010) Bifidobacterium longum lysate, a new ingredient for reactive skin. Exp Dermatol 19(8):E1–E8. https://doi.org/10.1111/j.1600-0625.2009.00932.x
doi: 10.1111/j.1600-0625.2009.00932.x
pubmed: 19624730
Gueniche A, Perin O et al (2022) Advances in microbiome-derived solutions and methodologies are founding a new era in skin health and care. Pathogens 11(2):121. https://doi.org/10.3390/pathogens11020121
doi: 10.3390/pathogens11020121
pubmed: 35215065
pmcid: 8879973
Hermo L, Smith CE (2011) Thirsty business: cell, region, and membrane specificity of aquaporins in the testis, efferent ducts, and epididymis and factors regulating their expression. J Androl 32(6):565–575. https://doi.org/10.2164/jandrol.110.012831
doi: 10.2164/jandrol.110.012831
pubmed: 21441426
Hong K, Jeong M et al (2015) Photoprotective effects of galacto-oligosaccharide and/or Bifidobacterium longum supplementation against skin damage induced by ultraviolet irradiation in hairless mice. Int J Food Sci Nutr 66(8):923–930. https://doi.org/10.3109/09637486.2015.1088823
doi: 10.3109/09637486.2015.1088823
pubmed: 26470918
Hong YK, An S et al (2022) Potential anti-ageing effects of probiotic-derived conditioned media on human skin cells. Acta Pharm 72(3):359–374. https://doi.org/10.2478/acph-2022-0027
doi: 10.2478/acph-2022-0027
pubmed: 36651546
Kakehi K, Kinoshita M et al (2003) Hyaluronic acid: separation and biological implications. J Chromatogr B Anal Technol Biomed Life Sci 797(1-2):347–355. https://doi.org/10.1016/S1570-0232(03)00479-3
doi: 10.1016/S1570-0232(03)00479-3
Kalliomaki M, Salminen S et al (2003) Probiotics and prevention of atopic disease: 4-year follow-up of a randomised placebo-controlled trial. Lancet 361(9372):1869–1871. https://doi.org/10.1016/S0140-6736(03)13490-3
doi: 10.1016/S0140-6736(03)13490-3
pubmed: 12788576
Karampoor M, Fouladpour A et al (2022) Probiotics as a promising treatment approach to burn wound healing. Burns 48(8). https://doi.org/10.1016/j.burns.2022.07.003
Kemgang TS, Kapila S et al (2014) Cross-talk between probiotic lactobacilli and host immune system. J Appl Microbiol 117(2):303–319. https://doi.org/10.1093/jn/137.3.781S
doi: 10.1093/jn/137.3.781S
pubmed: 24738909
Kenji K, Shunsuke S et al (2020) Vulnerability of the skin barrier to mechanical rubbing. Int J Pharm 587. https://doi.org/10.1016/j.ijpharm.2020.119708
Khmaladze I, Butler E et al (2019) Lactobacillus reuteri DSM 17938 - a comparative study on the effect of probiotics and lysates on human skin. Exp Dermatol 28(7):822–828. https://doi.org/10.1111/exd.13950
doi: 10.1111/exd.13950
pubmed: 31021014
Kim MY, Lim YY et al (2015) Synergistic inhibition of tumor necrosis factor-alpha-stimulated pro-inflammatory cytokine expression in HaCaT cells by a combination of rapamycin and mycophenolic acid. Ann Dermatol 27(1):32–39. https://doi.org/10.5021/ad.2015.27.1.32
doi: 10.5021/ad.2015.27.1.32
pubmed: 25673929
pmcid: 4323600
Kobayashi T, Chanmee T et al (2020) Hyaluronan: metabolism and function. Biomolecules 10(11). https://doi.org/10.3390/biom10111525
Lee H, Kim M (2022) Skin barrier function and the microbiome. Int J Mol Sci 23(21). https://doi.org/10.3390/ijms232113071
Lidbeck A, Nord CE (1991) Lactobacilli in relation to human-ecology and antimicrobial therapy. Int J Tissue React 13(2):115–122
pubmed: 1955293
Ma X, Pan Y et al (2022) Bifidobacteriumi nfantis strain YLGB-1496 possesses excellent antioxidant and skinbarrier-enhancing efficacy in vitro. Exp Dermatol 31(7):6. https://doi.org/10.1111/exd.14583
doi: 10.1111/exd.14583
Magdalena B, Ewa D et al (2016) Structural and biophysical characteristics of human skin in maintaining proper epidermal barrier function. Adv Dermatol Allergol 33(1). https://doi.org/10.5114/pdia.2015.48037
Mohtashami M, Mohamadi M et al (2020) Lactobacillus bulgaricus and Lactobacillus plantarum improve diabetic wound healing through modulating inflammatory factors. Biotechnol Appl Biochem 68(6). https://doi.org/10.1002/bab.2064
Moysidis M, Stavrou G et al (2022) The 3-D configuration of excisional skin wound healing after topical probiotic application. Injury 53(4). https://doi.org/10.1016/j.injury.2022.02.006
O'Callaghan A, van Sinderen D (2016) Bifidobacteria and their role as members of the human gut microbiota. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.00925
O'Neil DA (2003) Regulation of expression of beta-defensins: endogenous enteric peptide antibiotics. Mol Immunol 40(7):445–450. https://doi.org/10.1016/S0161-5890(03)00161-5
doi: 10.1016/S0161-5890(03)00161-5
pubmed: 14568390
Pandey KR, Naik SR et al (2015) Probiotics, prebiotics and synbiotics- a review. J Food Sci Technol 52(12):7577–7587. https://doi.org/10.1007/s13197-015-1921-1
doi: 10.1007/s13197-015-1921-1
pubmed: 26604335
pmcid: 4648921
Park MS, Kim JI et al (2018) Towards the application of human defensins as antivirals. Biomol Ther 26(3):13. https://doi.org/10.4062/biomolther.2017.172
doi: 10.4062/biomolther.2017.172
PueblaBarragan S, Reid G (2021) Probiotics in cosmetic and personal care products: trends and challenges. Molecules 26(5). https://doi.org/10.3390/molecules26051249
Rawal S, Ali SA (2023) Probiotics and postbiotics play a role in maintaining dermal health. Food Funct 14(9):3966–3981. https://doi.org/10.1039/D3FO00152K
doi: 10.1039/D3FO00152K
pubmed: 37051640
Ren D, Li C et al (2015) Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests. Anaerobe 35:22–27. https://doi.org/10.1016/j.anaerobe.2015.06.008
doi: 10.1016/j.anaerobe.2015.06.008
pubmed: 26143437
Rossi M, Amaretti A et al (2011) Folate production by probiotic bacteria. Nutrients 3(1):118–134. https://doi.org/10.3390/nu3010118
doi: 10.3390/nu3010118
pubmed: 22254078
pmcid: 3257725
Sen A, Nishimura T et al (2023) Comprehensive analysis of metabolites produced by co-cultivation of Bifidobacterium breve MCC1274 with human iPS-derived intestinal epithelial cells. Front Microbiol 14. https://doi.org/10.3389/fmicb.2023.1155438
Siiskonen H, Oikari S et al (2015) Hyaluronansynthase1: a mysterious enzyme with unexpected functions. Front Immunol 6:11. https://doi.org/10.3389/fimmu.2015.00043
doi: 10.3389/fimmu.2015.00043
Skandalis SS, Karalis T et al (2020) Intracellular hyaluronan: importance for cellular functions. Semin Cancer Biol 62:20–30. https://doi.org/10.1016/j.semcancer.2019.07.002
doi: 10.1016/j.semcancer.2019.07.002
pubmed: 31276783
Solopova A, Bottacini F et al (2020) Riboflavin biosynthesis and overproduction by a derivative of the human gut commensal Bifidobacterium longum subsp. infantis ATCC 15697. Front Microbiol 11:573335. https://doi.org/10.3389/fmicb.2020.573335
doi: 10.3389/fmicb.2020.573335
pubmed: 33042083
pmcid: 7522473
Spicer AP, Nguyen TK (1999) Mammalian hyaluronan synthases: investigation of functional relationships in vivo. Biochem Soc Trans 27(2):7. https://doi.org/10.1042/bst0270109
doi: 10.1042/bst0270109
Sroka-Tomaszewska J, Trzeciak M (2021) Molecular mechanisms of atopic dermatitis pathogenesis. Int J Mol Sci 22(8). https://doi.org/10.3390/ijms22084130
Suez J, Zmora N et al (2019) The pros, cons, and many unknowns of probiotics. Nat Med 25(5):716–729. https://doi.org/10.1038/s41591-019-0439-x
doi: 10.1038/s41591-019-0439-x
pubmed: 31061539
Takata K, Matsuzaki T et al (2004) Aquaporins: water channel proteins of the cellmembrane. Prog Histochem Cytochem 39(1):83. https://doi.org/10.1016/j.proghi.2004.03.001
doi: 10.1016/j.proghi.2004.03.001
Thorakkattu P, Khanashyam AC et al (2022) Postbiotics: current trends in food and pharmaceutical industry. Foods 11(19). https://doi.org/10.3390/foods11193094
Tsai W, Chou C et al (2021) Regulatory effects of Lactobacillus plantarum-GMNL6 on human skin health by improving skin microbiome. Int J Med Sci 18(5). https://doi.org/10.7150/ijms.51545
Vale ADS, de Melo Pereira GV et al (2023) Production, formulation, and application of postbiotics in the treatment of skin conditions. Fermentation 9(3). https://doi.org/10.3390/fermentation9030264
Vasiliki L, Mihalis IP (2019) Functional role of probiotics and prebiotics on skin health and disease. Fermentation 5(2). https://doi.org/10.3390/fermentation5020041
Wang F, Feng XC et al (2006) Aquaporins as potential drug targets. Acta Pharmacol Sin 27(4):395–401. https://doi.org/10.1111/j.1745-7254.2006.00318.x
doi: 10.1111/j.1745-7254.2006.00318.x
pubmed: 16539837
Wegh CAM, Geerlings SY et al (2019) Postbiotics and their potential applications in early life nutrition and beyond. Int J Mol Sci 20(19). https://doi.org/10.3390/ijms20194673
Wei Q, Chen T et al (2007) Using of lactobacillus and Bifidobacterium to product the isoflavone aglycones in fermented soymilk. Int J Food Microbiol 117(1):120–124. https://doi.org/10.1016/j.ijfoodmicro.2007.02.024
doi: 10.1016/j.ijfoodmicro.2007.02.024
pubmed: 17477997
Weigel PH (2002) Functional characteristics and catalytic mechanisms of the bacterial hyaluronan synthases. IUBMB Life 54(4):201–211. https://doi.org/10.1080/15216540214931
doi: 10.1080/15216540214931
pubmed: 12512859
Yano C, Saeki H et al (2015) Mechanism of macrophage-derived chemokine/CCL22 production by HaCaT keratinocytes. Ann Dermatol 27(2):152–156. https://doi.org/10.5021/ad.2015.27.2.152
doi: 10.5021/ad.2015.27.2.152
pubmed: 25834353
pmcid: 4377403
Yu J, Ma X et al (2022) Application and mechanism of probiotics in skin care: a review. J Cosmet Dermatol 21(3). https://doi.org/10.1111/jocd.14734
Zapaśnik A, Sokołowska B et al (2022) Role of lactic acid bacteria in food preservation and safety. Foods 11(9). https://doi.org/10.3390/foods11091283
Zhang Y, Hou Y et al (2023) Bifidobacterium animalis A12, a probiotic strain that promotes glucose and lipid metabolism, improved the texture and aroma of the fermented sausage. Foods 12(2). https://doi.org/10.3390/foods12020336
Zhao L, Wang S et al (2021) Identification, characterization, and antioxidant potential of Bifidobacterium longum subsp. longum strains isolated from feces of healthy infants. Front Microbiol 12. https://doi.org/10.3389/fmicb.2021.756519