Among the recombinant TSPOs, the BcTSPO.
Aminolevulinic acid
Functional recombinant TSPO
Hemin
Membrane protein
Porphyrin
Journal
Biochimie
ISSN: 1638-6183
Titre abrégé: Biochimie
Pays: France
ID NLM: 1264604
Informations de publication
Date de publication:
25 Jan 2024
25 Jan 2024
Historique:
received:
19
10
2023
revised:
17
01
2024
accepted:
18
01
2024
medline:
28
1
2024
pubmed:
28
1
2024
entrez:
27
1
2024
Statut:
aheadofprint
Résumé
Overexpression of recombinant Bacillus cereus TSPO (BcTSPO) in E. coli bacteria leads to its recovery with a bound hemin both in bacterial membrane (MB) and inclusion bodies (IB). Unlike mouse TSPO, BcTSPO purified in SDS detergent from IB is well structured and can bind various ligands such as high-affinity PK 11195, protoporphyrin IX (PPIX) and δ-aminolevulinic acid (ALA). For each of the three ligands,
Identifiants
pubmed: 38280504
pii: S0300-9084(24)00029-4
doi: 10.1016/j.biochi.2024.01.011
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2024. Published by Elsevier B.V.