Toxicological analyses of the venoms of Nigerian vipers Echis ocellatus and Bitis arietans.

Snake venom Snake venom metalloproteinases Snake venom phospholipase A2 Snake venom serine proteases Toxicological assay

Journal

Tropical medicine and health
ISSN: 1348-8945
Titre abrégé: Trop Med Health
Pays: Japan
ID NLM: 101215093

Informations de publication

Date de publication:
29 Jan 2024
Historique:
received: 30 11 2023
accepted: 16 01 2024
medline: 29 1 2024
pubmed: 29 1 2024
entrez: 28 1 2024
Statut: epublish

Résumé

Among the medically important snakes in Nigeria, Echis ocellatus and Bitis arietans have the most lethal venom. These venoms were classified according to the presence of snake venom metalloproteinases (SVMPs), snake venom phospholipase A Proteins were separated from venom using column chromatography. The skin and footpad of mice were used to determine hemorrhagic and edematogenic activities. Caprine blood plasma was used to test fibrinolytic activity in vitro. The results showed that, compared to the crude venom, the SVMP fraction induced hemorrhagic effects with a diameter of 26.00 ± 1.00 mm in E. ocellatus and 21.33 ± 1.52 mm in B. arietans. Both SVSP and SVMP had anticoagulant effects; however, the SVSP fraction had a stronger effect, with a longer anticoagulation time of 30.00 ± 3.00 min in E. ocellatus and 26.00 ± 2.00 min in B. arietans. These main venom toxins, SVMPs, SVSPs, and PLA Given the importance of SVMPs in altering the integrity of the membrane structure and impairing the blood coagulation system, an antivenom that can specifically neutralize its activity could inhibit the hemorrhage effects of the venoms.

Sections du résumé

BACKGROUND BACKGROUND
Among the medically important snakes in Nigeria, Echis ocellatus and Bitis arietans have the most lethal venom. These venoms were classified according to the presence of snake venom metalloproteinases (SVMPs), snake venom phospholipase A
METHODS METHODS
Proteins were separated from venom using column chromatography. The skin and footpad of mice were used to determine hemorrhagic and edematogenic activities. Caprine blood plasma was used to test fibrinolytic activity in vitro.
RESULTS RESULTS
The results showed that, compared to the crude venom, the SVMP fraction induced hemorrhagic effects with a diameter of 26.00 ± 1.00 mm in E. ocellatus and 21.33 ± 1.52 mm in B. arietans. Both SVSP and SVMP had anticoagulant effects; however, the SVSP fraction had a stronger effect, with a longer anticoagulation time of 30.00 ± 3.00 min in E. ocellatus and 26.00 ± 2.00 min in B. arietans. These main venom toxins, SVMPs, SVSPs, and PLA
CONCLUSIONS CONCLUSIONS
Given the importance of SVMPs in altering the integrity of the membrane structure and impairing the blood coagulation system, an antivenom that can specifically neutralize its activity could inhibit the hemorrhage effects of the venoms.

Identifiants

pubmed: 38282015
doi: 10.1186/s41182-024-00581-9
pii: 10.1186/s41182-024-00581-9
doi:

Types de publication

Journal Article

Langues

eng

Pagination

15

Informations de copyright

© 2024. The Author(s).

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Auteurs

Emeka John Dingwoke (EJ)

Department of Tropical Diseases, UNESCO-International Center for Biotechnology, University of Nigeria, Nsukka, Enugu State, Nigeria. dinhimself@yahoo.com.
Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria. dinhimself@yahoo.com.
Venom, Antivenom and Natural Toxins Research Centre, Ahmadu Bello University, Zaria, Kaduna State, Nigeria. dinhimself@yahoo.com.

Fatima Adis Adamude (FA)

Department of Biochemistry, Faculty of Sciences, Federal University, Lafia, Nasarawa State, Nigeria.
Venom, Antivenom and Natural Toxins Research Centre, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

Aliyu Salihu (A)

Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

Mujitaba Suleiman Abubakar (MS)

Department of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Venom, Antivenom and Natural Toxins Research Centre, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

Abdullahi Balarabe Sallau (AB)

Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria. bsallau@yahoo.com.
Venom, Antivenom and Natural Toxins Research Centre, Ahmadu Bello University, Zaria, Kaduna State, Nigeria. bsallau@yahoo.com.

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