Monitoring of hepatitis E virus in wastewater can identify clinically relevant variants.
hepatitis E virus
surveillance
treatment plants
wastewater
wastewater-based epidemiology
Journal
Liver international : official journal of the International Association for the Study of the Liver
ISSN: 1478-3231
Titre abrégé: Liver Int
Pays: United States
ID NLM: 101160857
Informations de publication
Date de publication:
30 Jan 2024
30 Jan 2024
Historique:
revised:
24
11
2023
received:
01
09
2023
accepted:
04
01
2024
medline:
31
1
2024
pubmed:
31
1
2024
entrez:
31
1
2024
Statut:
aheadofprint
Résumé
Hepatitis E virus (HEV) is prevalent worldwide and can cause persistent infection with severe morbidity. Antiviral treatment approaches can lead to the emergence of viral variants encoding escape mutations that may impede viral clearance. The frequency of these variants remains unknown in the human population as well as environment due to limited comprehensive data on HEV diversity. In this study, we investigated the HEV prevalence and diversity of circulating variants in environmental samples, that is, wastewater and rivers from North-Rhine Westphalia, Germany. HEV prevalence could be determined with 73% of samples tested positive for viral RNA via qRT-PCR. Using high-throughput sequencing, we were able to assess the overall genetic diversity in these samples and identified the presence of clinically relevant variants associated with drug resistance. In summary, monitoring variants from environmental samples could provide valuable insights into estimating HEV prevalence and identifying circulating variants that can impact treatment outcome.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : German Ministry of Education and Research
ID : HepEDiaSeq 01EK2106A/B
Organisme : German Ministry of Education and Research
ID : ViroBio01KI2106
Organisme : German Federal Ministry of Health
ID : ZMVI1-2518FSB705
Organisme : German Centre for Infection Research
Informations de copyright
© 2024 The Authors. Liver International published by John Wiley & Sons Ltd.
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