A SNP-based genotyping strategy to detect the abuse of homologous blood transfusion from dried blood spots.
genotyping
homologous blood transfusions
real time PCR
single nucleotide polymorphisms
sport doping
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
31 Jan 2024
31 Jan 2024
Historique:
revised:
28
12
2023
received:
09
08
2023
accepted:
04
01
2024
medline:
31
1
2024
pubmed:
31
1
2024
entrez:
31
1
2024
Statut:
aheadofprint
Résumé
We performed genotyping analysis of human biallelic polymorphisms (single nucleotide polymorphisms) for the detection of homologous blood transfusion in sports doping. DNA was extracted from dried blood spots and quantified real-time fast PCR. The method was proven to allow the detection of transfusions up to a donor percentage of 1%, with a significant improvement in terms of sensitivity with respect to both the reference cytofluorimetric method and a previously proposed strategy based on the DNA STR-based strategy.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
Références
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