A novel likely pathogenetic variant p.(Cys235Arg) of the MEN1 gene in multiple endocrine neoplasia type 1 with multifocal glucagonomas.

Adrenal gland adenoma Familiar Necrolytic migratory erythema Neuroendocrine pancreatic tumor Next-generation sequencing Primary hyperparathyroidism

Journal

Journal of endocrinological investigation
ISSN: 1720-8386
Titre abrégé: J Endocrinol Invest
Pays: Italy
ID NLM: 7806594

Informations de publication

Date de publication:
31 Jan 2024
Historique:
received: 26 06 2023
accepted: 17 12 2023
medline: 31 1 2024
pubmed: 31 1 2024
entrez: 31 1 2024
Statut: aheadofprint

Résumé

Multiple endocrine neoplasia type 1 (MEN1) is a hereditary endocrine syndrome caused by pathogenic variants in MEN1 tumor suppressor gene. Diagnosis is commonly based on clinical criteria and confirmed by genetic testing. The objective of the present study was to report on a MEN1 case characterized by multiple pancreatic glucagonomas, with particular concern on the possible predisposing genetic defects. While conducting an extensive review of the most recent scientific evidence on the unusual glucagonoma familial forms, we analyzed the MEN1 gene in a 35-year-old female with MEN1, as well as her son and daughter, using Sanger and next-generation sequencing (NGS) approaches. We additionally explored the functional and structural consequences of the identified variant using in silico analyses. NGS did not show any known pathogenic variant in the tested regions. However, a new non-conservative variant in exon 4 of MEN1 gene was found in heterozygosity in the patient and in her daughter, resulting in an amino acid substitution from hydrophobic cysteine to hydrophilic arginine at c.703T > C, p.(Cys235Arg). This variant is absent from populations databases and was never reported in full papers: its characteristics, together with the high specificity of the patient's clinical phenotype, pointed toward a possible causative role. Our findings confirm the need for careful genetic analysis of patients with MEN1 and establish a likely pathogenic role for the new p.(Cys235Arg) variant, at least in the rare subset of MEN1 associated with glucagonomas.

Identifiants

pubmed: 38294658
doi: 10.1007/s40618-023-02287-x
pii: 10.1007/s40618-023-02287-x
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s).

Références

Thakker RV, Newey PJ, Walls GV et al (2012) Clinical practice guidelines for multiple endocrine neoplasia type 1 (MEN1). J Clin Endocrinol Metab 97:2990–3011. https://doi.org/10.1210/jc.2012-1230
doi: 10.1210/jc.2012-1230 pubmed: 22723327
Brandi ML, Gagel RF, Angeli A et al (2001) Guidelines for diagnosis and therapy of MEN type 1 and type 2. J Clin Endocrinol Metab 86:5658–5671. https://doi.org/10.1210/jcem.86.12.8070
doi: 10.1210/jcem.86.12.8070 pubmed: 11739416
Febrero B, Segura P, Ruiz-Manzanera JJ et al (2021) Uncommon tumors in multiple endocrine neoplasia (MEN) type 1: do they have a relationship with the prognosis of these patients? J Endocrinol Investig 44:1327–1330. https://doi.org/10.1007/s40618-020-01414-2
doi: 10.1007/s40618-020-01414-2
Chandrasekharappa SC, Guru SC, Manickam P et al (1997) Positional cloning of the gene for multiple endocrine neoplasia-type 1. Science 276:404–407. https://doi.org/10.1126/science.276.5311.404
doi: 10.1126/science.276.5311.404 pubmed: 9103196
Rizzoli R, Green J, Marx SJ (1985) Primary hyperparathyroidism in familial multiple endocrine neoplasia Type I. Long-term follow-up of serum calcium levels after parathyroidectomy. Am J Med 78:467–474. https://doi.org/10.1016/0002-9343(85)90340-7
doi: 10.1016/0002-9343(85)90340-7 pubmed: 2858157
Giraud S, Zhang CX, Serova-Sinilnikova O et al (1998) Germ-line mutation analysis in patients with multiple endocrine neoplasia Type 1 and related disorders. Am J Hum Genet 63:455–467. https://doi.org/10.1086/301953
doi: 10.1086/301953 pubmed: 9683585 pmcid: 1377295
Figueiredo AA, Saramago A, Cavaco BM et al (2023) Familial parathyroid tumours-comparison of clinical profiles between syndromes. J Endocrinol Investig 46:1799–1806. https://doi.org/10.1007/s40618-023-02032-4
doi: 10.1007/s40618-023-02032-4
Larsson C, Skogseid B, Oberg K et al (1988) Multiple endocrine neoplasia type 1 gene maps to chromosome 11 and is lost in insulinoma. Nature 332:85–87. https://doi.org/10.1038/332085a0
doi: 10.1038/332085a0 pubmed: 2894610
Bassett JH, Forbes SA, Pannett AA et al (1998) Characterization of mutations in patients with multiple endocrine neoplasia type 1. Am J Hum Genet 62:232–244. https://doi.org/10.1086/301729
doi: 10.1086/301729 pubmed: 9463336 pmcid: 1376903
Agarwal SK, Lee Burns A, Sukhodolets KE et al (2004) Molecular pathology of the MEN1 gene. Ann N Y Acad Sci 1014:189–198. https://doi.org/10.1196/annals.1294.020
doi: 10.1196/annals.1294.020 pubmed: 15153434
Lemos MC, Thakker RV (2008) Multiple endocrine neoplasia type 1 (MEN1): analysis of 1336 mutations reported in the first decade following identification of the gene. Hum Mutat 29:22–32. https://doi.org/10.1002/humu.20605
doi: 10.1002/humu.20605 pubmed: 17879353
Ruggeri RM, Benevento E, De Cicco F et al (2022) Neuroendocrine neoplasms in the context of inherited tumor syndromes: a reappraisal focused on targeted therapies. J Endocrinol Investig 46:213–234. https://doi.org/10.1007/s40618-022-01905-4
doi: 10.1007/s40618-022-01905-4
Newey PJ, Newell-Price J (2022) MEN1 Surveillance Guidelines: time to (Re)Think? J Endocr Soc 6:bvac01. https://doi.org/10.1210/jendso/bvac001
doi: 10.1210/jendso/bvac001
Lavezzi E, Brunetti A, Smiroldo V et al (2022) Case report: new CDKN1B mutation in multiple endocrine neoplasia Type 4 and brief literature review on clinical management. Front Endocrinol (Lausanne) 13:773143. https://doi.org/10.3389/fendo.2022.773143
doi: 10.3389/fendo.2022.773143 pubmed: 35355569
Richards S, Aziz N, Bale S et al (2015) Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med 17:405–424. https://doi.org/10.1038/gim.2015.30
doi: 10.1038/gim.2015.30 pubmed: 25741868 pmcid: 4544753
Kelley LA, Mezulis S, Yates CM et al (2015) The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc 10:845–858. https://doi.org/10.1038/nprot.2015.053
doi: 10.1038/nprot.2015.053 pubmed: 25950237 pmcid: 5298202
Kopanos C, Tsiolkas V, Kouris A et al (2019) VarSome: the human genomic variant search engine. Bioinformatics 35:1978–1980. https://doi.org/10.1093/bioinformatics/bty897
doi: 10.1093/bioinformatics/bty897 pubmed: 30376034
Ayala-Ramirez M, Feng L, Johnson MM et al (2011) Clinical risk factors for malignancy and overall survival in patients with pheochromocytomas and sympathetic paragangliomas: primary tumor size and primary tumor location as prognostic indicators. J Clin Endocrinol Metab 96:717–725. https://doi.org/10.1210/jc.2010-194
doi: 10.1210/jc.2010-194 pubmed: 21190975
Newey PJ, Thakker RV (2011) Role of multiple endocrine neoplasia type 1 mutational analysis in clinical practice. Endocr Pract 17:8–17. https://doi.org/10.4158/EP10379.RA
doi: 10.4158/EP10379.RA pubmed: 21454234
Jin S, Mao H, Schnepp RW et al (2003) Menin associates with FANCD2, a protein involved in repair of DNA damage. Cancer Res 63:4204–4210
pubmed: 12874027
Asgharian B, Turner ML, Gibril F et al (2004) Cutaneous tumors in patients with multiple endocrine neoplasm type 1 (MEN1) and gastrinomas: prospective study of frequency and development of criteria with high sensitivity and specificity for MEN1. J Clin Endocrinol Metab 89:5328–5336. https://doi.org/10.1210/jc.2004-0218
doi: 10.1210/jc.2004-0218 pubmed: 15531478
Kindmark H, Sundin A, Granberg D et al (2007) Endocrine pancreatic tumors with glucagon hypersecretion: a retrospective study of 23 cases during 20 years. Med Oncol 24:330–337. https://doi.org/10.1007/s12032-007-0011-2
doi: 10.1007/s12032-007-0011-2 pubmed: 17873310
Halfdanarson TR, Rubin J, Farnell MB et al (2008) Pancreatic endocrine neoplasms: epidemiology and prognosis of pancreatic endocrine tumors. Endocr Relat Cancer 15:409–427. https://doi.org/10.1677/ERC-07-0221
doi: 10.1677/ERC-07-0221 pubmed: 18508996 pmcid: 2693313
Yao JC, Eisner MP, Leary C et al (2007) Population-based study of islet cell carcinoma. Ann Surg Oncol 14:3492–3500. https://doi.org/10.1245/s10434-007-9566-6
doi: 10.1245/s10434-007-9566-6 pubmed: 17896148 pmcid: 2077912
Öberg K (2015) Neuroendocrine gastro-enteropancreatic tumors - from eminence based to evidence-based medicine—a Scandinavian view. Scand J Gastroenterol 50:727–739. https://doi.org/10.3109/00365521.2015.1033001
doi: 10.3109/00365521.2015.1033001 pubmed: 25855088
de Herder WW, Hofland J (2023) Glucagon & Glucagonoma Syndrome. In: Feingold KR, Anawalt B, Blackman MR et al (eds). Endotext [Internet]. MDText.com, South Dartmouth (MA, USA). https://www.ncbi.nlm.nih.gov/books/NBK279041/ . Accessed 29 Nov 2023
Sandhu S, Jialal I (2022) Glucagonoma syndrome. In: StatPearls [Internet]. StatPearls Publishing, Treasure Island (FL, USA). https://www.ncbi.nlm.nih.gov/books/NBK519500/ . Accessed 29 Nov 2023
Adam DN, Cohen PD, Ghazarian D (2003) Necrolytic migratory erythema: case report and clinical review. J Cutan Med Surg 7:333–338. https://doi.org/10.1007/s10227-002-0127-0
doi: 10.1007/s10227-002-0127-0 pubmed: 14738101
Sawicki MP, Wan YJ, Johnson CL et al (1992) Loss of heterozygosity on chromosome 11 in sporadic gastrinomas. Hum Genet 89:445–449. https://doi.org/10.1007/BF00194320
doi: 10.1007/BF00194320 pubmed: 1352275
Hessman O, Lindberg D, Skogseid B et al (1998) Mutation of the multiple endocrine neoplasia type 1 gene in nonfamilial, malignant tumors of the endocrine pancreas. Cancer Res 58:377–379
pubmed: 9458074
Sarui H, Yoshimoto K, Okumura S et al (1997) Cystic glucagonoma with loss of heterozygosity on chromosome 11 in multiple endocrine neoplasia type 1. Clin Endocrinol (Oxf) 46:511–516. https://doi.org/10.1046/j.1365-2265.1997.1380965.x
doi: 10.1046/j.1365-2265.1997.1380965.x pubmed: 9196616
Lévy-Bohbot N, Merle C, Goudet P et al (2004) Prevalence, characteristics and prognosis of MEN 1-associated glucagonomas, VIPomas, and somatostatinomas: study from the GTE (Groupe des Tumeurs Endocrines) registry. Gastroenterol Clin Biol 28:1075–1081. https://doi.org/10.1016/s0399-8320(04)95184-6
doi: 10.1016/s0399-8320(04)95184-6 pubmed: 15657529
Ro C, Chai W, Yu VE et al (2013) Pancreatic neuroendocrine tumors: biology, diagnosis, and treatment. Chin J Cancer 32:312–324. https://doi.org/10.5732/cjc.012.10295
doi: 10.5732/cjc.012.10295 pubmed: 23237225 pmcid: 3845620
Boden G, Owen OE (1977) Familial hyperglucagonemia—an autosomal dominant disorder. N Engl J Med 296:534–538. https://doi.org/10.1056/NEJM197703102961003
doi: 10.1056/NEJM197703102961003 pubmed: 189188
Stacpoole PW, Jaspan J, Kasselberg AG et al (1981) A familial glucagonoma syndrome: genetic, clinical and biochemical features. Am J Med 70:1017–1026. https://doi.org/10.1016/0002-9343(81)90858-5
doi: 10.1016/0002-9343(81)90858-5 pubmed: 6112877
Murakami T, Usui T, Nakajima A et al (2015) A novel missense mutation of the MEN1 gene in a patient with multiple endocrine neoplasia Type 1 with glucagonoma and obesity. Intern Med 54:2475–2481. https://doi.org/10.2169/internalmedicine.54.4886
doi: 10.2169/internalmedicine.54.4886 pubmed: 26424307
Erdas E, Aste N, Pilloni L et al (2012) Functioning glucagonoma associated with primary hyperparathyroidism: multiple endocrine neoplasia type 1 or incidental association? BMC Cancer 12:614. https://doi.org/10.1186/1471-2407-12-614
doi: 10.1186/1471-2407-12-614 pubmed: 23259638 pmcid: 3543729
Nishiuchi T, Imachi H, Murao K et al (2009) Co-existence of glucagonoma with recurrent insulinoma in a patient with multiple endocrine neoplasia-type 1 (MEN-1). Endocrine 36:20–24. https://doi.org/10.1007/s12020-009-9183-1
doi: 10.1007/s12020-009-9183-1 pubmed: 19350420
Chen X, Sun S, Wang C et al (2015) Novel mutation 928G>C of MEN1 gene in a familial multiple endocrine neoplasia type 1 case (MEN1) with co-existence of insulinoma and glucagonoma. J Diabetes 7:426–429. https://doi.org/10.1111/1753-0407.12199
doi: 10.1111/1753-0407.12199 pubmed: 25047095
Cho YY, Chung YJ (2021) A germline c. 1546dupC MEN1 mutation in an MEN1 family: a case report. Medicine (Baltimore) 100(25):e26382. https://doi.org/10.1097/MD.0000000000026382
doi: 10.1097/MD.0000000000026382 pubmed: 34160414
Scarpa A, Chang DK, Nones K et al (2017) Whole-genome landscape of pancreatic neuroendocrine tumours. Nature 543:65–71. https://doi.org/10.1038/nature21063
doi: 10.1038/nature21063 pubmed: 28199314
Stacpoole PW (1981) The glucagonoma syndrome: clinical features, diagnosis, and treatment. Endocr Rev 2:347–361. https://doi.org/10.1210/edrv-2-3-347
doi: 10.1210/edrv-2-3-347 pubmed: 6268399
Brandi ML, Agarwal SK, Perrier ND et al (2021) Multiple endocrine neoplasia type 1: latest insights. Endocr Rev 42:133–170. https://doi.org/10.1210/endrev/bnaa031
doi: 10.1210/endrev/bnaa031 pubmed: 33249439
Falchetti A, Marini F, Luzi E et al (2009) Multiple endocrine neoplasia type 1 (MEN1): not only inherited endocrine tumors. Genet Med 11:825–835. https://doi.org/10.1097/GIM.0b013e3181be5c97
doi: 10.1097/GIM.0b013e3181be5c97 pubmed: 19904212

Auteurs

C Smirne (C)

Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100, Novara, Italy. carlo.smirne@med.uniupo.it.
Division of Internal Medicine, University Hospital Maggiore della Carità, 28100, Novara, Italy. carlo.smirne@med.uniupo.it.

G M Giacomini (GM)

Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100, Novara, Italy.
Division of Internal Medicine, University Hospital Maggiore della Carità, 28100, Novara, Italy.

A M Berton (AM)

Division of Endocrinology, Diabetes and Metabolism, City of Health and Science University Hospital, 10126, Turin, Italy.

B Pasini (B)

Department of Medical Sciences, University of Turin, 10126, Turin, Italy.
Division of Medical Genetics, City of Health and Science University Hospital, 10126, Turin, Italy.

F Mercalli (F)

Division of Pathology, University Hospital Maggiore della Carità, 28100, Novara, Italy.

F Prodam (F)

Department of Health Sciences, University of Piemonte Orientale, 28100, Novara, Italy.
Division of Endocrinology, University Hospital Maggiore della Carità, 28100, Novara, Italy.

M Caputo (M)

Department of Health Sciences, University of Piemonte Orientale, 28100, Novara, Italy.
Division of Endocrinology, University Hospital Maggiore della Carità, 28100, Novara, Italy.

L A A Brosens (LAA)

Department of Pathology, University Medical Center Utrecht, 3584 CX, Utrecht, The Netherlands.

E L M Mollero (ELM)

Division of Endocrinology, University Hospital Maggiore della Carità, 28100, Novara, Italy.

R Pitino (R)

Division of Endocrinology, University Hospital Maggiore della Carità, 28100, Novara, Italy.

M Pirisi (M)

Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100, Novara, Italy.
Division of Internal Medicine, University Hospital Maggiore della Carità, 28100, Novara, Italy.

G Aimaretti (G)

Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100, Novara, Italy.
Division of Endocrinology, University Hospital Maggiore della Carità, 28100, Novara, Italy.

E Ghigo (E)

Division of Endocrinology, Diabetes and Metabolism, City of Health and Science University Hospital, 10126, Turin, Italy.
Department of Medical Sciences, University of Turin, 10126, Turin, Italy.

Classifications MeSH