In vitro and In vivo Effects of Ethanolic Extract of Fumaria parviflora Lam. Embedded in Chitosan Nanoparticles Against Leishmania major.

Chitosan nanoparticle Drug delivery Fumaria parviflora Lam Leishmania major Wound healing

Journal

Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947

Informations de publication

Date de publication:
31 Jan 2024
Historique:
received: 04 03 2023
accepted: 12 12 2023
medline: 31 1 2024
pubmed: 31 1 2024
entrez: 31 1 2024
Statut: aheadofprint

Résumé

Fumaria has been traditionally used to treat skin damages due to anti-inflammatory properties. In the present study, we evaluated the effect of the ethanolic extract of Fumaria parviflora Lam. (F. parviflora) against Leishmania major (L. major) using chitosan biopolymer drug delivery system both In vitro and In vivo models. The ethanolic extract of F. parviflora was analyzed by HPLC to determine its active ingredients content. The extract was then loaded on chitosan nanoparticles (CNPs). The parasite was treated with various concentrations of the ethanolic extract, CNPs and CNPs loaded with F. parviflora extract (CNPs@ F. parviflora). The size of lesions of treated mice were measured on a weekly basis. The parasite burden was evaluated 8 weeks after treatment. The HPLC analysis showed the presence of Fumaric acid at a high concentration. The percentage of the drug released from CNPs@ F. parviflora within 24 and 72 h were 65% and 90% respectively. The results showed that F. parviflora extract and CNPs@ F. parviflora caused 84% and 96% growth inhibition of L. major promastigotes as revealed by Neubauer chamber counting and MTT test respectively. The IC50 values of F. parviflora extract and CNPs@ F. parviflora were 450 and 68.4 µg/ml respectively. In amastigote assay, the best results showed in CNPs@ F. parviflora that only 2% of macrophages were infected with amastigotes. In vivo experiments for mice treated with F. parviflora and CNPs @ F. parviflora in comparison to control group showed a significant reduction (P < 0.05) in the mean diameter of the lesions (2.3 and 1.72 mm and 9.91 mm respectively). The ethanolic extract of F. parviflora both as standalone and loaded in CNPs showed promising inhibitory effects against L. major both upon In vitro and In vivo experimentation as well as therapeutic effects for wound healing in infected mice.

Identifiants

DOI: 10.1007/s11686-023-00784-w PMID: 38294710
pubmed: 38294710
doi: 10.1007/s11686-023-00784-w
pii: 10.1007/s11686-023-00784-w
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Références

Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, Boer M (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7(5):e35671. https://doi.org/10.1371/journal.pone.0035671
doi: 10.1371/journal.pone.0035671 pubmed: 22693548 pmcid: 3365071
Sundar S, More DK, Singh MK, Singh VP, Sharma S, Makharia A, Kumar PC, Murray HW (2000) Failure of pentavalent antimony in visceral leishmaniasis in India: report from the center of the Indian epidemic. Clin Infect Dis 31(4):1104–1107. https://doi.org/10.1086/318121
doi: 10.1086/318121 pubmed: 11049798
Beheshti N, Soflaeia S, Shakibaieb M, Yazdi MH, Ghaffarifar F, Dalimi A, Shahverdi AR (2013) Efficacy of biogenic selenium nanoparticles against Leishmania major: In vitro and In vivo studies. J Trace Elem Med Biol 27(3):203–207. https://doi.org/10.1016/j.jtemb.2012.11.002
doi: 10.1016/j.jtemb.2012.11.002 pubmed: 23219368
Ghaffarifar F, Jorjani O, Sharifi Z, Dalimi A, Hassan ZM, Tabatabaie F, Khoshzaban F, Hezarjaribi HZ (2013) Enhancement of immune response induced by DNA vaccine cocktail expressing complete LACK and TSA genes against Leishmania major. APMIS 121:290–298. https://doi.org/10.1111/j.1600-0463.2012.02968.x
doi: 10.1111/j.1600-0463.2012.02968.x pubmed: 23030526
Ghaffarifar F (2018) Plasmid DNA vaccines: where are we now. Drugs Today 54:315–333. https://doi.org/10.1358/dot.2018.54.5.2807864
doi: 10.1358/dot.2018.54.5.2807864
Ghaffarifar F, Heydari F, Dalimi A, Hassan ZM, Delavari M, Mikaeiloo H (2015) Evaluation of apoptotic and antileishmanial activities of Artemisinin on promastigotes and BALB/C mice infected with Leishmania major. Iran J Parasitol 10:258
pubmed: 26246824 pmcid: 4522302
Kumar S, Sharma NK, Kamboj A (2017) Fumaria parviflora Lam. (Fumitory): a traditional herbal medicine with modern evidence. Asian J Pharm Sci 3(6):200–207
Chauhan NS (1999). Medicinal and aromatic plants of Himachal Pradesh, Indus publishing.
Orhan IE, Şener B, Musharraf SG (2012) Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp Toxicol Pathol 64(3):205–209. https://doi.org/10.1016/j.etp.2010.08.007
doi: 10.1016/j.etp.2010.08.007 pubmed: 20829009
Botanical Society of Britain and Ireland. Archived from the original (xls) on 2015–01–25. Retrieved 2014–10–17.
Mozaffarian V (1994) Dictionary of Iranian plant names: Latin, English, Persian. Published by Farhang Moaser. pp 238.[In Persian]
Zargari A (1997) Medicinal plants, seventh edition. Tehran Institute of Publishing and University, 1, pp 72–166 (In Persian)
Contreras M, Bribi N, Gómez-Caravaca AM, Gálvez J, Segura-Carretero A (2017) Alkaloids profiling of fumaria capreolata by analytical platforms based on the hyphenation of gas chromatography and liquid chromatography with quadrupole-time-of-flight mass spectrometry. Int J Anal Chem. https://doi.org/10.1155/2017/5178729
doi: 10.1155/2017/5178729 pubmed: 29348751 pmcid: 5733964
Suau R, Cabezudo B, Rico R, Lopez-Romero JM (2002) Direct determination of alkaloid contents in Fumaria species by GC-MS. Phytochem Anal Int J Plant Chem Biochem Tech 13(6):363–367. https://doi.org/10.1002/pca.669
doi: 10.1002/pca.669
Jameel M, Islamuddin M, Ali A, Afrin F, Ali M (2014) Isolation, characterization and antimicrobial evaluation of a novel compound N-octacosan 7 β ol, from Fumaria parviflora Lam. BMC Complement Med Ther 14(1):98
doi: 10.1186/1472-6882-14-98
Jowkar F, Jamshidzadeh A, Yazdi AM, Pasalar M (2011) The effects of fumaria parviflora L. extract on chronic hand eczema: a randomized double-blind placebo controlled clinical trial. Iran Red Crescent Med J 13(11):824
pubmed: 22737422 pmcid: 3371888
Orhan IE, Öztürk N, Sener B (2015) Antiprotozoal assessment and phenolic acid profiling of five Fumaria (fumitory) species. Asian Pac J Trop Med 8(4):283–286. https://doi.org/10.1016/S1995-7645(14)60331-X
doi: 10.1016/S1995-7645(14)60331-X pubmed: 25975499
Hördegen P, Hertzberg H, Heilmann J, Maurer V (2003) The anthelmintic efficacy of five plant products against gastrointestinal trichostrongylids in artificially infected lambs. Vet Parasitol 117(1–2):51–60. https://doi.org/10.1016/j.vetpar.2003.07.027
doi: 10.1016/j.vetpar.2003.07.027 pubmed: 14597279
Benita S (2005) Microencapsulation: methods and industrial applications. CRC Press
Jayakumar R, Nwe N, Tokura S, Tamura H (2007) Sulfated chitin and chitosan as novel biomaterials. Int J Biol Macromol 40(3):175–181. https://doi.org/10.1016/j.ijbiomac.2006.06.021
doi: 10.1016/j.ijbiomac.2006.06.021 pubmed: 16893564
Rinaudo M (2008) Main properties and current applications of some polysaccharides as biomaterials. Polym Int 57(3):397–430. https://doi.org/10.1002/pi.2378
doi: 10.1002/pi.2378
Mourya V, Inamdar NN (2008) Chitosan-modifications and applications: opportunities galore. React Funct Polym 68(6):1013–1051. https://doi.org/10.1016/j.reactfunctpolym.2008.03.002
doi: 10.1016/j.reactfunctpolym.2008.03.002
Alishahi A, Mirvaghefi A, Rafiee Tehrani M, Farahmand H, Koshio S, Dorkoosh FA, Elsabeé MZ (2011) Chitosan nanoparticle to carry vitamin C through the gastrointestinal tract and induce the non-specific immunity system of rainbow trout (Oncorhynchus mykiss). Carbohydr Polym 86(1):142–146. https://doi.org/10.1016/j.carbpol.2011.04.028
doi: 10.1016/j.carbpol.2011.04.028
Chaubey P, Mishra B, Mudavath SL, Patel RR, Chaurasia S, Sundar S, Suvarna V, Monteiro M (2018) Mannose-conjugated curcumin-chitosan nanoparticles: efficacy and toxicity assessments against Leishmania donovani. Int J Biol Macromol 111:109–120
doi: 10.1016/j.ijbiomac.2017.12.143 pubmed: 29307805
Qi L, Xu Z (2004) Lead sorption from aqueous solutions on chitosan nanoparticles. Coll Surf A Physicochem Eng 251(1–3):183–190. https://doi.org/10.1016/j.colsurfa.2004.10.010
doi: 10.1016/j.colsurfa.2004.10.010
Karimipoursaryazdi A, Ghaffarifar F, Dalimi A, Dayer MS (2020) In-vitro and in-vivo comparative effects of the spring and autumn-harvested Artemisia aucheri Bioss extracts on Leishmania major. J Ethnopharmacol. https://doi.org/10.1016/j.jep.2020.112910
doi: 10.1016/j.jep.2020.112910 pubmed: 32344159
Ebrahimisadr P, Ghaffarifar F, Horton J, Dalimi A, Sharifi Z (2018) Apoptotic effect of morphine, imiquimod and nalmefene on promastigote, infected and uninfected macrophages with amastigote of Leishmania major by flow cytometry. Iran J Pharm Res 17(3):986–994
pubmed: 30127821 pmcid: 6094415
Azimbeyk M, Mousavi Gargari SL, Nazarian S (2017) Chitosan nanogel based candidate vaccine against ehec. J Adv Med Biomed Res 25(109):85–99
Mukherjee D, Yousuf M, Dey S, Chakraborty S, Chaudhuri A, Kumar V, Sarkar B, Nath S, Hussain A, Dutta A, Mishra T, Biswajit BG, Singh S, Chakraborty S, Adhikari S, Pal C (2020) Targeting the trypanothione reductase of tissue-residing leishmania in hosts’ reticuloendothelial system a flexible water-soluble ferrocenylquinoline-based preclinical drug candidate. J Med Chem 63(24):15621–15638. https://doi.org/10.1021/acs.jmedchem.0c00690
doi: 10.1021/acs.jmedchem.0c00690 pubmed: 33296601
Thompson AM, O’Connor PD, Marshall AJ (2021) Heteroaryl ether analogues of an antileishmanial 7-substituted 2-nitroimidazooxazine lead afford attenuated hERG risk: In vitro and In vivo appraisal. Eur J Med Chem 209:112914. https://doi.org/10.1016/j.ejmech.2020.112914
doi: 10.1016/j.ejmech.2020.112914 pubmed: 33268145
Simin A, Ghaffarifar F, Delavari H, Bineshian F (2021) Evaluation the effects of Fumaria parviflora aqueous extracts on Leishmania major In vitro and In vivo. Pathobiol Res 23(5):37–45

Auteurs

Azar Simin (A)

Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Islamic Republic of Iran.

Fatemeh Ghaffarifar (F)

Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Islamic Republic of Iran. ghafarif@modares.ac.ir.
ORCID: 0000-0003-0891-8214

Hamid Delavari (H)

Department of Materials Engineering, Nanomaterials Group, Tarbiat Modares University, Tehran, Iran.

Mohammad Saaid Dayer (MS)

Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Islamic Republic of Iran.

Najla Hamidianfar (N)

Department of Natural Resources and Environmental Sciences, Faculty of Agriculture and Natural Resources, Islamic Azad University Khorasgan (Isfahan) Branch, Isfahan, Islamic Republic of Iran.

Farzaneh Baghkhani (F)

Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Islamic Republic of Iran.

Classifications MeSH