New insights on collagen structural organization and spatial distribution around dental implants: a comparison between machined and laser-treated surfaces.

Collagen Dental implants Fourier Transform InfraRed Imaging spectroscopy Healing abutments Implant surface Synthegra Transmucosal surface

Journal

Journal of translational medicine
ISSN: 1479-5876
Titre abrégé: J Transl Med
Pays: England
ID NLM: 101190741

Informations de publication

Date de publication:
31 Jan 2024
Historique:
received: 23 06 2023
accepted: 18 01 2024
medline: 1 2 2024
pubmed: 1 2 2024
entrez: 31 1 2024
Statut: epublish

Résumé

One of the main factors for the osseointegration of dental implants is the development of an adequate soft tissue barrier, mainly composed by collagen, which protects the implant from bacterial development. The structural features of the peri-implant collagen are influenced by the implant components and, in particular, by the type of the surface. In the clinical practice, healing abutments are characterized by smooth surfaces, named machined. Recently, a new laser technique, Synthegra, has been developed to obtain a topography-controlled surface with micrometric regular pores that seems reducing the risk of peri-implantitis. Based on this background, this study aims investigating the structural organization and spatial distribution of collagen surrounding healing abutments characterized by laser-treated and machined surfaces. Gingiva portions surrounding custom-made healing abutments (HA), characterized by alternated laser-treated and machined surfaces, were collected and analyzed by combining Fourier Transform InfraRed Imaging (FTIRI) spectroscopy, a non-invasive and high-resolution bidimensional analytical technique, with histological and multivariate analyses. Masson's trichrome staining, specific for collagen, highlighted a massive presence of collagen in all the analyzed samples, evidencing a surface-related spatial distribution. The nature of collagen, investigated by the FTIRI spectroscopy, appeared more abundant close to the laser-treated surface, with a perpendicular disposition of the bundles respect to the HA; conversely, a parallel distribution was observed around the machined surface. A different secondary structure was also found, with a higher amount of triple helices and a lower quantity of random coils in collagen close to the laser treated surfaces. FTIRI spectroscopy demonstrates that the use of a laser treated transmucosal surface can improve the morphological organization of the peri-implant collagen, which presents a distribution more similar to that of natural teeth. This trial is registered with ClinicalTrials.gov Identifier: (Registration Number: NCT05754970). Registered 06/03/2023, retrospectively registered, https://clinicaltrials.gov/show/NCT05754970 .

Sections du résumé

BACKGROUND BACKGROUND
One of the main factors for the osseointegration of dental implants is the development of an adequate soft tissue barrier, mainly composed by collagen, which protects the implant from bacterial development. The structural features of the peri-implant collagen are influenced by the implant components and, in particular, by the type of the surface. In the clinical practice, healing abutments are characterized by smooth surfaces, named machined. Recently, a new laser technique, Synthegra, has been developed to obtain a topography-controlled surface with micrometric regular pores that seems reducing the risk of peri-implantitis. Based on this background, this study aims investigating the structural organization and spatial distribution of collagen surrounding healing abutments characterized by laser-treated and machined surfaces.
METHODS METHODS
Gingiva portions surrounding custom-made healing abutments (HA), characterized by alternated laser-treated and machined surfaces, were collected and analyzed by combining Fourier Transform InfraRed Imaging (FTIRI) spectroscopy, a non-invasive and high-resolution bidimensional analytical technique, with histological and multivariate analyses.
RESULTS RESULTS
Masson's trichrome staining, specific for collagen, highlighted a massive presence of collagen in all the analyzed samples, evidencing a surface-related spatial distribution. The nature of collagen, investigated by the FTIRI spectroscopy, appeared more abundant close to the laser-treated surface, with a perpendicular disposition of the bundles respect to the HA; conversely, a parallel distribution was observed around the machined surface. A different secondary structure was also found, with a higher amount of triple helices and a lower quantity of random coils in collagen close to the laser treated surfaces.
CONCLUSIONS CONCLUSIONS
FTIRI spectroscopy demonstrates that the use of a laser treated transmucosal surface can improve the morphological organization of the peri-implant collagen, which presents a distribution more similar to that of natural teeth.
TRIAL REGISTRATION BACKGROUND
This trial is registered with ClinicalTrials.gov Identifier: (Registration Number: NCT05754970). Registered 06/03/2023, retrospectively registered, https://clinicaltrials.gov/show/NCT05754970 .

Identifiants

DOI: 10.1186/s12967-024-04906-4 PMID: 38297308
pubmed: 38297308
doi: 10.1186/s12967-024-04906-4
pii: 10.1186/s12967-024-04906-4
doi:

Banques de données

ClinicalTrials.gov
['NCT05754970']

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

120

Subventions

Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : ex 60% 2022 B.S.

Informations de copyright

© 2024. The Author(s).

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Auteurs

Alessia Belloni (A)

Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.

Giulio Argentieri (G)

Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.

Giulia Orilisi (G)

Department of Clinical Sciences and Stomatology, Polytechnic University of Marche, Ancona, Italy.

Valentina Notarstefano (V)

Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.

Elisabetta Giorgini (E)

Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.

Gianmaria D'Addazio (G)

Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.

Giovanna Orsini (G)

Department of Clinical Sciences and Stomatology, Polytechnic University of Marche, Ancona, Italy. g.orsini@staff.univpm.it.
ORCID: 0000-0002-2641-0695

Sergio Caputi (S)

Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.

Bruna Sinjari (B)

Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.

Classifications MeSH