Evolution and function analysis of auxin response factors reveal the molecular basis of the developed root system of Zygophyllum xanthoxylum.
A-ZxARFs
Auxin response factors
Lateral roots
ZxARFs
Zygophyllum xanthoxylum
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
02 Feb 2024
02 Feb 2024
Historique:
received:
20
11
2023
accepted:
29
12
2023
medline:
2
2
2024
pubmed:
2
2
2024
entrez:
2
2
2024
Statut:
epublish
Résumé
As a xerophytic shrub, forming developed root system dominated with lateral roots is one of the effective strategies for Zygophyllum xanthoxylum to adapt to desert habitat. However, the molecular mechanism of lateral root formation in Z. xanthoxylum is still unclear. Auxin response factors (ARFs) are a master family of transcription factors (TFs) in auxin-mediated biological processes including root growth and development. Here, to determine the relationship between ARFs and root system formation in Z. xanthoxylum, a total of 30 potential ZxARF genes were first identified, and their classifications, evolutionary relationships, duplication events and conserved domains were characterized. 107 ARF protein sequences from alga to higher plant species including Z. xanthoxylum are split into A, B, and C 3 Clades, consisting with previous studies. The comparative analysis of ARFs between xerophytes and mesophytes showed that A-ARFs of xerophytes expanded considerably more than that of mesophytes. Furthermore, in this Clade, ZxARF5b and ZxARF8b have lost the important B3 DNA-binding domain partly and completely, suggesting both two proteins may be more functional in activating transcription by dimerization with AUX/IAA repressors. qRT-PCR results showed that all A-ZxARFs are high expressed in the roots of Z. xanthoxylum, and they were significantly induced by drought stress. Among these A-ZxARFs, the over-expression assay showed that ZxARF7c and ZxARF7d play positive roles in lateral root formation. This study provided the first comprehensive overview of ZxARFs and highlighted the importance of A-ZxARFs in the lateral root development.
Sections du résumé
BACKGROUND
BACKGROUND
As a xerophytic shrub, forming developed root system dominated with lateral roots is one of the effective strategies for Zygophyllum xanthoxylum to adapt to desert habitat. However, the molecular mechanism of lateral root formation in Z. xanthoxylum is still unclear. Auxin response factors (ARFs) are a master family of transcription factors (TFs) in auxin-mediated biological processes including root growth and development.
RESULTS
RESULTS
Here, to determine the relationship between ARFs and root system formation in Z. xanthoxylum, a total of 30 potential ZxARF genes were first identified, and their classifications, evolutionary relationships, duplication events and conserved domains were characterized. 107 ARF protein sequences from alga to higher plant species including Z. xanthoxylum are split into A, B, and C 3 Clades, consisting with previous studies. The comparative analysis of ARFs between xerophytes and mesophytes showed that A-ARFs of xerophytes expanded considerably more than that of mesophytes. Furthermore, in this Clade, ZxARF5b and ZxARF8b have lost the important B3 DNA-binding domain partly and completely, suggesting both two proteins may be more functional in activating transcription by dimerization with AUX/IAA repressors. qRT-PCR results showed that all A-ZxARFs are high expressed in the roots of Z. xanthoxylum, and they were significantly induced by drought stress. Among these A-ZxARFs, the over-expression assay showed that ZxARF7c and ZxARF7d play positive roles in lateral root formation.
CONCLUSION
CONCLUSIONS
This study provided the first comprehensive overview of ZxARFs and highlighted the importance of A-ZxARFs in the lateral root development.
Identifiants
pubmed: 38302884
doi: 10.1186/s12870-023-04717-7
pii: 10.1186/s12870-023-04717-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
81Subventions
Organisme : National Natural Science Foundation of China
ID : 31971621
Organisme : National Natural Science Foundation of China
ID : 31971621
Organisme : National Natural Science Foundation of China
ID : 31971621
Organisme : National Natural Science Foundation of China
ID : 31971621
Organisme : National Natural Science Foundation of China
ID : 31971621
Organisme : China Postdoctoral Science Foundation
ID : 2023M741495
Organisme : China Postdoctoral Science Foundation
ID : 2023M741495
Organisme : China Postdoctoral Science Foundation
ID : 2023M741495
Organisme : China Postdoctoral Science Foundation
ID : 2023M741495
Organisme : China Postdoctoral Science Foundation
ID : 2023M741495
Organisme : Key Science & Technology Project of Gansu Province
ID : 22ZD6NA007
Organisme : Key Science & Technology Project of Gansu Province
ID : 22ZD6NA007
Organisme : Key Science & Technology Project of Gansu Province
ID : 22ZD6NA007
Organisme : Key Science & Technology Project of Gansu Province
ID : 22ZD6NA007
Organisme : Key Science & Technology Project of Gansu Province
ID : 22ZD6NA007
Informations de copyright
© 2024. The Author(s).
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