NRF2 attenuation aggravates detrimental consequences of metabolic stress on cultured porcine parthenote embryos.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
05 Feb 2024
Historique:
received: 16 09 2023
accepted: 31 01 2024
medline: 6 2 2024
pubmed: 6 2 2024
entrez: 5 2 2024
Statut: epublish

Résumé

The nuclear factor erythroid 2-related factor 2 (NRF2) is a crucial transcription factor that plays a central role in regulating oxidative stress pathways by binding antioxidant response elements, but its involvement in early embryo development remains largely unexplored. In this study, we demonstrated that NRF2 mRNA is expressed in porcine embryos from day 2 to day 7 of development, showing a decrease in abundance from day 2 to day 3, followed by an increase on day 5 and day 7. Comparable levels of NRF2 mRNA were observed between early-cleaving and more developmental competent embryos and late-cleaving and less developmental competent embryos on day 4 and day 5 of culture. Attenuation of NRF2 mRNA significantly decreased development of parthenote embryos to the blastocyst stage. When NRF2-attenuated embryos were cultured in presence of 3.5 mM or 7 mM glucose, development to the blastocyst stage was dramatically decreased in comparison to the control group (15.9% vs. 27.8% for 3.5 mM glucose, and 5.4% vs. 25.3% for 7 mM glucose). Supplementation of melatonin moderately improved the development of NRF2-attenuated embryos cultured in presence of 0.6 mM glucose. These findings highlight the importance of NRF2 in early embryo development, particularly in embryos cultured under metabolically stressful conditions.

Identifiants

pubmed: 38316940
doi: 10.1038/s41598-024-53480-8
pii: 10.1038/s41598-024-53480-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2973

Informations de copyright

© 2024. The Author(s).

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Auteurs

Werner Giehl Glanzner (WG)

Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte Anne de Bellevue, QC, H9X 3V9, Canada. werner.glanzner@mcgill.ca.

Leticia Rabello da Silva Sousa (LR)

Veterinary Medicine Department, College of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, SP, Brazil.

Karina Gutierrez (K)

Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte Anne de Bellevue, QC, H9X 3V9, Canada.

Mariana Priotto de Macedo (MP)

Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte Anne de Bellevue, QC, H9X 3V9, Canada.

Luke Currin (L)

Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte Anne de Bellevue, QC, H9X 3V9, Canada.

Felipe Perecin (F)

Veterinary Medicine Department, College of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, SP, Brazil.

Vilceu Bordignon (V)

Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte Anne de Bellevue, QC, H9X 3V9, Canada. vilceu.bordignon@mcgill.ca.

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