Experimental transmission of a novel relapsing fever group Borrelia harbored by Ornithodoros octodontus (Ixodida: Argasidae) in Chile.
MLST
Phylogeny
Relapsing fever
Soft-tick-borne diseases
Vector competence
“Candidatus Borrelia octodonta”
Journal
Experimental & applied acarology
ISSN: 1572-9702
Titre abrégé: Exp Appl Acarol
Pays: Netherlands
ID NLM: 8507436
Informations de publication
Date de publication:
06 Feb 2024
06 Feb 2024
Historique:
received:
10
10
2023
accepted:
29
12
2023
medline:
7
2
2024
pubmed:
7
2
2024
entrez:
6
2
2024
Statut:
aheadofprint
Résumé
Tick-borne relapsing fever spirochetes of genus Borrelia thrive in enzootic cycles involving Ornithodoros spp. (Argasidae) mainly, and rodents. The isolation of these spirochetes usually involves a murine model in which ticks are fed and the spirochetes detected in blood several days later. Such an experiment also demonstrates that a given species of tick is competent in the transmission of the bacteria. Here, soft ticks Ornithodoros octodontus were collected in Northern Chile with the objective to experimentally determine its capacity to transmit a Borrelia sp. detected in a previous study. Two Guinea pigs (Cavia porcellus) were used to feed nymphs and adults of O. octodontus and the spirochetes in blood were inspected by dark-field microscopy and nested PCR. Although spirochetes were not seen in blood, DNA was detected in only one animal 11 days after the ticks were fed. Genetic sequences of Borrelia flaB, clpX, pepX, recG, rplB, and uvrA genes retrieved from DNA extraction of positive blood were employed to construct two phylogenetic analyses. On the one hand, the flaB tree showed the Borrelia sp. transmitted by O. octodontus clustering with Borrelia sp. Alcohuaz, which was previously detected in that same tick species. On the other hand, concatenated clpX-pepX-recG-rplB-uvrA demonstrated that the characterized spirochete branches together with "Candidatus Borrelia caatinga", a recently discovered species from Brazil. Based on the genetic profile presented in this study, the name "Candidatus Borrelia octodonta" is proposed for the species transmitted by O. octodontus. The fact that spirochetes were not observed in blood of guinea pigs, may reflect the occurrence of low spirochetemia, which could be explained because the susceptibility of infection varies depending on the rodent species that is used in experimental models. Although the vertebrate reservoir of "Ca. Borrelia octodonta" is still unknown, Octodon degus, a rodent species that is commonly parasitized by O. octodontus, should be a future target to elucidate this issue.
Identifiants
pubmed: 38321309
doi: 10.1007/s10493-023-00881-5
pii: 10.1007/s10493-023-00881-5
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agencia Nacional de Investigación y Desarrollo
ID : DOCTORADO NACIONAL/2019-21190078
Organisme : Agencia Nacional de Investigación y Desarrollo
ID : DOCTORADO NACIONAL/2020-21200182
Organisme : Fondo Nacional de Desarrollo Científico y Tecnológico
ID : 11220177
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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