Racemization of the substrate and product by serine palmitoyltransferase from Sphingobacterium multivorum yields two enantiomers of the product from D-serine.

PLP-dependent enzyme X-ray crystallography crystal structure racemization serine palmitoyltransferase sphingolipid

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
05 Feb 2024
Historique:
received: 24 12 2023
revised: 25 01 2024
accepted: 02 02 2024
medline: 8 2 2024
pubmed: 8 2 2024
entrez: 7 2 2024
Statut: aheadofprint

Résumé

Serine palmitoyltransferase (SPT) catalyzes the pyridoxal-5'-phosphate (PLP)-dependent decarboxylative condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (KDS). Although SPT was shown to synthesize corresponding products from amino acids other than L-serine, it is still arguable whether SPT catalyzes the reaction with D-serine, which is a question of biological importance. Using high substrate and enzyme concentrations, KDS was detected after the incubation of SPT from Sphingobacterium multivorum with D-serine and palmitoyl-CoA. Furthermore, the KDS comprised equal amounts of 2S- and 2R-isomers.

Identifiants

DOI: 10.1016/j.jbc.2024.105728 PMID: 38325740
pubmed: 38325740
pii: S0021-9258(24)00104-2
doi: 10.1016/j.jbc.2024.105728
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

105728

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.

Auteurs

Hiroko Ikushiro (H)

Department of Biochemistry, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan. Electronic address: hiroko.ikushiro@ompu.ac.jp.

Takumi Honda (T)

Graduate School of Life Science, Hokkaido University, Kita 21, Nishi 11, Kita-ku, Sapporo 001-0021, Japan.

Yuta Murai (Y)

Graduate School of Life Science, Hokkaido University, Kita 21, Nishi 11, Kita-ku, Sapporo 001-0021, Japan; Frontier Research Center for Advanced Material and Life Science, Faculty of Advanced Life Science, Hokkaido University, Kita21 Nishi11, Sapporo, Hokkaido 001-0021, Japan; Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita9 Nishi9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan. Electronic address: ymurai@agr.hokudai.ac.jp.

Taiki Murakami (T)

Department of Chemistry, Graduate School of Science, Osaka Metropolitan University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, Osaka 558-8585, Japan.

Aya Takahashi (A)

Department of Chemistry, Graduate School of Science, Osaka Metropolitan University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, Osaka 558-8585, Japan.

Taiki Sawai (T)

Department of Biochemistry, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan.

Haruna Goto (H)

Department of Biochemistry, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan.

Shin-Ichi Ikushiro (SI)

Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.

Ikuko Miyahara (I)

Department of Chemistry, Graduate School of Science, Osaka Metropolitan University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, Osaka 558-8585, Japan.

Yoshio Hirabayashi (Y)

RIKEN Cluster for Pioneering Research, RIKEN, Wako, Saitama 351-0198, Japan; Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Chiba 279-0021, Japan.

Nobuo Kamiya (N)

Research Center for Artificial Photosynthesis, Osaka Metropolitan University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, Osaka 558-8585, Japan.

Kenji Monde (K)

Graduate School of Life Science, Hokkaido University, Kita 21, Nishi 11, Kita-ku, Sapporo 001-0021, Japan; Frontier Research Center for Advanced Material and Life Science, Faculty of Advanced Life Science, Hokkaido University, Kita21 Nishi11, Sapporo, Hokkaido 001-0021, Japan. Electronic address: kmonde@sci.hokudai.ac.jp.

Takato Yano (T)

Department of Biochemistry, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan. Electronic address: takato.yano@ompu.ac.jp.

Classifications MeSH