Kidney resident macrophages have distinct subsets and multifunctional roles.

Kidney RNA-sequencing basement membrane cytometry extracellular matrix macrophages proteomics

Journal

Matrix biology : journal of the International Society for Matrix Biology
ISSN: 1569-1802
Titre abrégé: Matrix Biol
Pays: Netherlands
ID NLM: 9432592

Informations de publication

Date de publication:
06 Feb 2024
Historique:
received: 15 06 2023
revised: 02 02 2024
accepted: 05 02 2024
medline: 9 2 2024
pubmed: 9 2 2024
entrez: 8 2 2024
Statut: aheadofprint

Résumé

The kidney contains distinct glomerular and tubulointerstitial compartments with diverse cell types and extracellular matrix components. The role of immune cells in glomerular environment is crucial for dampening inflammation and maintaining homeostasis. Macrophages are innate immune cells that are influenced by their tissue microenvironment. However, the multifunctional role of kidney macrophages remains unclear. Flow and imaging cytometry were used to determine the relative expression of CD81 and CX In wild type mice we identified three distinct kidney macrophage subsets using CD81 and CX We identified CD81 and CX

Sections du résumé

BACKGROUND BACKGROUND
The kidney contains distinct glomerular and tubulointerstitial compartments with diverse cell types and extracellular matrix components. The role of immune cells in glomerular environment is crucial for dampening inflammation and maintaining homeostasis. Macrophages are innate immune cells that are influenced by their tissue microenvironment. However, the multifunctional role of kidney macrophages remains unclear.
METHODS METHODS
Flow and imaging cytometry were used to determine the relative expression of CD81 and CX
RESULTS RESULTS
In wild type mice we identified three distinct kidney macrophage subsets using CD81 and CX
CONCLUSIONS CONCLUSIONS
We identified CD81 and CX

Identifiants

DOI: 10.1016/j.matbio.2024.02.002 PMID: 38331051
pubmed: 38331051
pii: S0945-053X(24)00021-0
doi: 10.1016/j.matbio.2024.02.002
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no competing financial disclosures.

Auteurs

Christine Chew (C)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK; Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK.

Oliver J Brand (OJ)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Tomohiko Yamamura (T)

Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK.

Craig Lawless (C)

Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK.

Mychel Raony Paiva Teixeira Morais (MRPT)

Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK.

Leo Zeef (L)

Bioinformatics Core Facility, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

I-Hsuan Lin (IH)

Bioinformatics Core Facility, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Gareth Howell (G)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Sylvia Lui (S)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Franziska Lausecker (F)

Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK.

Christopher Jagger (C)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Tovah N Shaw (TN)

Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, Edinburgh, EH9 3FL, UK.

Siddharth Krishnan (S)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Flora A McClure (FA)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Hayley Bridgeman (H)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Kelly Wemyss (K)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Joanne E Konkel (JE)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK.

Tracy Hussell (T)

Lydia Becker Institute for Immunology and Inflammation, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, M13 9PL, UK. Electronic address: Tracy.Hussell@manchester.ac.uk.

Rachel Lennon (R)

Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK; Department of Paediatric Nephrology, Royal Manchester Children's Hospital, Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, M13 9WL, UK. Electronic address: Rachel.Lennon@manchester.ac.uk.

Classifications MeSH