Endothelium-mediated regulation of platelet activation: Involvement of multiple protein kinases.
endothelial cells
glycoprotein VI
inter-cellular cross-talk
phosphoproteome
platelets
protease-activated receptors
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
29 Feb 2024
29 Feb 2024
Historique:
revised:
11
01
2024
received:
25
02
2023
accepted:
23
01
2024
medline:
9
2
2024
pubmed:
9
2
2024
entrez:
9
2
2024
Statut:
ppublish
Résumé
The endothelial regulation of platelet activity is incompletely understood. Here we describe novel approaches to find molecular pathways implicated on the platelet-endothelium interaction. Using high-shear whole-blood microfluidics, employing coagulant or non-coagulant conditions at physiological temperature, we observed that the presence of human umbilical vein endothelial cells (HUVEC) strongly suppressed platelet adhesion and activation, via the collagen receptor glycoprotein VI (GPVI) and the PAR receptors for thrombin. Real-time monitoring of the cytosolic Ca
Identifiants
pubmed: 38334433
doi: 10.1096/fj.202300360RR
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23468Subventions
Organisme : EC | Horizon 2020 Framework Programme (H2020)
ID : 766118
Organisme : EC | Horizon 2020 Framework Programme (H2020)
ID : 813409
Informations de copyright
© 2024 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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