PIEZO1 is essential for the survival and proliferation of acute myeloid leukemia cells.

PIEZO1 acute myeloid leukemia apoptosis cell cycle proliferation survival

Journal

Cancer medicine
ISSN: 2045-7634
Titre abrégé: Cancer Med
Pays: United States
ID NLM: 101595310

Informations de publication

Date de publication:
Jan 2024
Historique:
revised: 06 01 2024
received: 27 10 2023
accepted: 19 01 2024
medline: 9 2 2024
pubmed: 9 2 2024
entrez: 9 2 2024
Statut: ppublish

Résumé

Leukemogenesis is a complex process that interconnects tumoral cells with their microenvironment, but the effect of mechanosensing in acute myeloid leukemia (AML) blasts is poorly known. PIEZO1 perceives and transmits the constraints of the environment to human cells by acting as a non-selective calcium channel, but very little is known about its role in leukemogenesis. For the first time, we show that PIEZO1 is preferentially expressed in healthy hematopoietic stem and progenitor cells in human hematopoiesis, and globally overexpressed in AML cells. In AML subtypes, PIEZO1 expression associates with favorable outcomes as better overall (OS) and disease-free survival (DFS). If PIEZO1 is expressed and functional in THP1 leukemic myeloid cell line, its chemical activation doesn't impact the proliferation, differentiation, nor survival of cells. However, the downregulation of PIEZO1 expression dramatically reduces the proliferation and the survival of THP1 cells. We show that PIEZO1 knock-down blocks the cell cycle in G0/G1 phases of AML cells, impairs the DNA damage response pathways, and critically increases cell death by triggering extrinsic apoptosis pathways. Altogether, our results reveal a new role for PIEZO1 mechanosensing in the survival and proliferation of leukemic blasts, which could pave the way for new therapeutic strategies to target AML cells.

Identifiants

pubmed: 38334477
doi: 10.1002/cam4.6984
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e6984

Subventions

Organisme : Ligue Contre le Cancer
Organisme : JNCL

Informations de copyright

© 2024 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

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Auteurs

Delphine Lebon (D)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.
Hématologie Clinique et Thérapie Cellulaire, CHU Amiens-Picardie, Amiens, France.

Louison Collet (L)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.
Hématologie Clinique et Thérapie Cellulaire, CHU Amiens-Picardie, Amiens, France.

Stefan Djordjevic (S)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.

Cathy Gomila (C)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.

Hakim Ouled-Haddou (H)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.

Jessica Platon (J)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.

Yohann Demont (Y)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.
Service d'Hématologie Biologie, CHU Amiens-Picardie, Amiens, France.

Jean-Pierre Marolleau (JP)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.
Hématologie Clinique et Thérapie Cellulaire, CHU Amiens-Picardie, Amiens, France.

Alexis Caulier (A)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.
Division of Hematology/Oncology Boston Children's Hospital, Boston, Massachusetts, USA.
Department of Medical and Population Genetics, The Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.

Loïc Garçon (L)

HEMATIM UR4666, Université Picardie Jules Verne, Amiens, France.
Service d'Hématologie Biologie, CHU Amiens-Picardie, Amiens, France.

Classifications MeSH