OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells.

Humans Optogenetics Phytochrome B / genetics T-Lymphocytes / metabolism Receptors, Antigen, T-Cell / genetics Cell Differentiation Light
T cell receptor T cells extracellular optogenetics phytochromes primary cells receptor activation

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
15 Mar 2024
Historique:
medline: 18 3 2024
pubmed: 9 2 2024
entrez: 9 2 2024
Statut: ppublish

Résumé

Optogenetics is a versatile and powerful tool for the control and analysis of cellular signaling processes. The activation of cellular receptors by light using optogenetic switches usually requires genetic manipulation of cells. However, this considerably limits the application in primary, nonengineered cells, which is crucial for the study of physiological signaling processes and for controlling cell fate and function for therapeutic purposes. To overcome this limitation, we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT (

Identifiants

DOI: 10.1021/acssynbio.3c00518 PMID: 38335541
pubmed: 38335541
doi: 10.1021/acssynbio.3c00518
doi:

Substances chimiques

Phytochrome B 136250-22-1
Receptors, Antigen, T-Cell 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

752-762

Auteurs

Anja Armbruster (A)

INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken 66123, Germany.
Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.

Anna K Ehret (AK)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.
Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstr. 19A, Freiburg 79104, Germany.

Marissa Russ (M)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.

Vincent Idstein (V)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.
Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstr. 19A, Freiburg 79104, Germany.
ORCID: 0009-0007-8594-7752

Melissa Klenzendorf (M)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.

Denise Gaspar (D)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.

Claudia Juraske (C)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.
Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstr. 19A, Freiburg 79104, Germany.

O Sascha Yousefi (OS)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.

Wolfgang W Schamel (WW)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.

Wilfried Weber (W)

INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken 66123, Germany.
Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.
Department of Materials Science and Engineering, Saarland University, Campus D2 2, Saarbrücken 66123, Germany.

Maximilian Hörner (M)

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.
Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › OptoREACT: Optogenetic Receptor Activation on...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Optogénétique › OptoREACT: Optogenetic Receptor Activation on...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Phytochrome › Phytochrome B › OptoREACT: Optogenetic Receptor Activation on...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › OptoREACT: Optogenetic Receptor Activation on...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs aux antigènes › Récepteurs aux antigènes des cellules T › OptoREACT: Optogenetic Receptor Activation on...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › OptoREACT: Optogenetic Receptor Activation on...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › OptoREACT: Optogenetic Receptor Activation on...