Resilience and proteome response of Escherichia coli to high levels of isoleucine mistranslation.
Chaperone
Isoleucyl-tRNA synthetase
Mistranslation
Norvaline
Protein aggregation
Valine
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
Mar 2024
Mar 2024
Historique:
received:
13
09
2023
revised:
12
01
2024
accepted:
07
02
2024
pubmed:
11
2
2024
medline:
11
2
2024
entrez:
10
2
2024
Statut:
ppublish
Résumé
Accurate pairing of amino acids and tRNAs is a prerequisite for faithful translation of genetic information during protein biosynthesis. Here we present the effects of proteome-wide mistranslation of isoleucine (Ile) by canonical valine (Val) or non-proteinogenic norvaline (Nva) in a genetically engineered Escherichia coli strain with an editing-defective isoleucyl-tRNA synthetase (IleRS). Editing-defective IleRS efficiently mischarges both Val and Nva to tRNA
Identifiants
pubmed: 38340920
pii: S0141-8130(24)00871-7
doi: 10.1016/j.ijbiomac.2024.130068
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
130068Informations de copyright
Copyright © 2024 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.