The activity of antimicrobial peptoids against multidrug-resistant ocular pathogens.

Antimicrobial peptoids Keratitis Pseudomonas aeruginosa Staphylococcus aureus

Journal

Contact lens & anterior eye : the journal of the British Contact Lens Association
ISSN: 1476-5411
Titre abrégé: Cont Lens Anterior Eye
Pays: England
ID NLM: 9712714

Informations de publication

Date de publication:
09 Feb 2024
Historique:
received: 27 07 2023
revised: 11 01 2024
accepted: 04 02 2024
medline: 11 2 2024
pubmed: 11 2 2024
entrez: 10 2 2024
Statut: aheadofprint

Résumé

Ocular infections caused by antibiotic-resistant pathogens can result in partial or complete vision loss. The development of pan-resistant microbial strains poses a significant challenge for clinicians as there are limited antimicrobial options available. Synthetic peptoids, which are sequence-specific oligo-N-substituted glycines, offer potential as alternative antimicrobial agents to target multidrug-resistant bacteria. The antimicrobial activity of synthesised peptoids against multidrug-resistant (MDR) ocular pathogens was evaluated using the microbroth dilution method. Hemolytic propensity was assessed using mammalian erythrocytes. Peptoids were also incubated with proteolytic enzymes, after which their minimum inhibitory activity against bacteria was re-evaluated. Several alkylated and brominated peptoids showed good inhibitory activity against multidrug-resistant Pseudomonas aeruginosa strains at concentrations of ≤15 μg mL Peptoids studied here demonstrated potent activity against various multidrug-resistant ocular pathogens. Their properties make them promising candidates for controlling vision-related morbidity associated with eye infections by antibiotic-resistant strains.

Sections du résumé

BACKGROUND BACKGROUND
Ocular infections caused by antibiotic-resistant pathogens can result in partial or complete vision loss. The development of pan-resistant microbial strains poses a significant challenge for clinicians as there are limited antimicrobial options available. Synthetic peptoids, which are sequence-specific oligo-N-substituted glycines, offer potential as alternative antimicrobial agents to target multidrug-resistant bacteria.
METHODS METHODS
The antimicrobial activity of synthesised peptoids against multidrug-resistant (MDR) ocular pathogens was evaluated using the microbroth dilution method. Hemolytic propensity was assessed using mammalian erythrocytes. Peptoids were also incubated with proteolytic enzymes, after which their minimum inhibitory activity against bacteria was re-evaluated.
RESULTS RESULTS
Several alkylated and brominated peptoids showed good inhibitory activity against multidrug-resistant Pseudomonas aeruginosa strains at concentrations of ≤15 μg mL
CONCLUSIONS CONCLUSIONS
Peptoids studied here demonstrated potent activity against various multidrug-resistant ocular pathogens. Their properties make them promising candidates for controlling vision-related morbidity associated with eye infections by antibiotic-resistant strains.

Identifiants

pubmed: 38341309
pii: S1367-0484(24)00007-9
doi: 10.1016/j.clae.2024.102124
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102124

Informations de copyright

Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Manjulatha Sara (M)

School of Optometry and Vision Science, UNSW Sydney, Australia. Electronic address: manjulatha.sara@unsw.edu.au.

Muhammad Yasir (M)

School of Optometry and Vision Science, UNSW Sydney, Australia.

Parthasarathi Kalaiselvan (P)

School of Optometry and Vision Science, UNSW Sydney, Australia.

Alex Hui (A)

School of Optometry and Vision Science, UNSW Sydney, Australia; Centre for Ocular Research and Education, University of Waterloo, Canada.

Rajesh Kuppusamy (R)

School of Optometry and Vision Science, UNSW Sydney, Australia; School of Chemistry, UNSW Sydney, Australia.

Naresh Kumar (N)

School of Chemistry, UNSW Sydney, Australia.

Sudip Chakraborty (S)

School of Chemistry, UNSW Sydney, Australia.

Tsz Tin Yu (TT)

School of Chemistry, UNSW Sydney, Australia.

Edgar H H Wong (EHH)

School of Chemical Engineering, UNSW Sydney, Australia.

Natalia Molchanova (N)

The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 4720, USA.

Håvard Jenssen (H)

Department of Science and Environment, Roskilde University, 4000 Roskilde, Denmark.

Jennifer S Lin (JS)

Department of Bioengineering, School of Medicine & School of Engineering, Stanford University, Stanford, CA 9430, USA.

Annelise E Barron (AE)

Department of Bioengineering, School of Medicine & School of Engineering, Stanford University, Stanford, CA 9430, USA.

Mark Willcox (M)

School of Optometry and Vision Science, UNSW Sydney, Australia. Electronic address: m.willcox@unsw.edu.au.

Classifications MeSH