Three-dimensional topography of rat trigeminal ganglion neurons using a combination of retrograde labeling and tissue-clearing techniques.
3DISCO
orofacial region
retrograde tracing
sensory nerve
three-dimensional reconstruction
tissue transparency
trigeminal ganglion
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
Feb 2024
Feb 2024
Historique:
revised:
28
12
2023
received:
07
09
2023
accepted:
09
01
2024
medline:
11
2
2024
pubmed:
11
2
2024
entrez:
11
2
2024
Statut:
ppublish
Résumé
The trigeminal nerve is the sensory afferent of the orofacial regions and divided into three major branches. Cell bodies of the trigeminal nerve lie in the trigeminal ganglion and are surrounded by satellite cells. There is a close interaction between ganglion cells via satellite cells, but the function is not fully understood. In the present study, we clarified the ganglion cells' three-dimensional (3D) localization, which is essential to understand the functions of cell-cell interactions in the trigeminal ganglion. Fast blue was injected into 12 sites of the rat orofacial regions, and ganglion cells were retrogradely labeled. The labeled trigeminal ganglia were cleared by modified 3DISCO, imaged with confocal laser-scanning microscopy, and reconstructed in 3D. Histograms of the major axes of the fast blue-positive somata revealed that the peak major axes of the cells innervating the skin/mucosa were smaller than those of cells innervating the deep structures. Ganglion cells innervating the ophthalmic, maxillary, and mandibular divisions were distributed in the anterodorsal, central, and posterolateral portions of the trigeminal ganglion, respectively, with considerable overlap in the border region. The intermingling in the distribution of ganglion cells within each division was also high, in particular, within the mandibular division. Specifically, intermingling was observed in combinations of tongue and masseter/temporal muscles, maxillary/mandibular molars and masseter/temporal muscles, and tongue and mandibular molars. Double retrograde labeling confirmed that some ganglion cells innervating these combinations were closely apposed. Our data provide essential information for understanding the function of ganglion cell-cell interactions via satellite cells.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e25584Subventions
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP23H03119
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP23K09316
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP19K10058
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP19K10336
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP19KK0419
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP22H05162
Organisme : Grants-in-Aid from The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP22K09916
Informations de copyright
© 2024 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC.
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