Protocol to investigate Parkinson's patient-derived dopaminergic neurons by live-cell microscopy and oxidized dopamine assays.
Cell Biology
Neuroscience
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
15 Mar 2024
15 Mar 2024
Historique:
received:
02
11
2023
revised:
12
12
2023
accepted:
29
01
2024
pubmed:
11
2
2024
medline:
11
2
2024
entrez:
11
2
2024
Statut:
ppublish
Résumé
Dopaminergic neurons derived from human induced pluripotent stem cells recapitulate key pathogenic phenotypes observed in Parkinson's disease (PD). Here, we present a protocol to analyze oxidized dopamine and the recruitment of parkin onto synaptic vesicles in neurons derived from patients with mutations in parkin that cause autosomal recessive PD. We describe steps for neuronal differentiation, live-cell microscopy, detection of oxidized dopamine, and labeling of synaptic vesicles. These protocols can be applied to studies of other forms of genetic and sporadic forms of PD. For complete details on the use and execution of this protocol, please refer to Song et al.
Identifiants
pubmed: 38341851
pii: S2666-1667(24)00054-6
doi: 10.1016/j.xpro.2024.102889
pmc: PMC10867435
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102889Subventions
Organisme : NCI NIH HHS
ID : P30 CA060553
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS076054
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS122257
Pays : United States
Organisme : NINDS NIH HHS
ID : R37 NS096241
Pays : United States
Informations de copyright
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.