Deciphering Membrane-Protein Interactions and High-Throughput Antigen Identification with Cell Doublets.
T cell antigen discovery
TCR
doublets
membrane-protein interaction
pMHC
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
11 Feb 2024
11 Feb 2024
Historique:
revised:
02
01
2024
received:
16
08
2023
medline:
12
2
2024
pubmed:
12
2
2024
entrez:
11
2
2024
Statut:
aheadofprint
Résumé
Deciphering cellular interactions is essential to both understand the mechanisms underlying a broad range of human diseases, but also to manipulate therapies targeting these diseases. Here, the formation of cell doublets resulting from specific membrane ligand-receptor interactions is discovered. Based on this phenomenon, the study developed DoubletSeeker, a novel high-throughput method for the reliable identification of ligand-receptor interactions. The study shows that DoubletSeeker can accurately identify T cell receptor (TCR)-antigen interactions with high sensitivity and specificity. Notably, DoubletSeeker effectively captured paired TCR-peptide major histocompatibility complex (pMHC) information during a highly complex library-on-library screening and successfully identified three mutant TCRs that specifically recognize the MART-1 epitope. In turn, DoubletSeeker can act as an antigen discovery platform that allows for the development of novel immunotherapy targets, making it valuable for investigating fundamental tumor immunology.
Identifiants
pubmed: 38342599
doi: 10.1002/advs.202305750
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2305750Subventions
Organisme : National Natural Science Foundation of China
ID : 81972875
Organisme : National Natural Science Foundation of China
ID : 32270994
Organisme : Natural Science Foundation Outstanding Youth Fund of Jiangsu Province
ID : BK20211505
Organisme : Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences
ID : 2021-RC310-014
Organisme : Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences
ID : 2019PT310028
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2023-I2M-QJ-019
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-047
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-061
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2022-I2M-2-004
Organisme : Gusu Innovation and Entrepreneurship Talent Program
ID : ZXL2022503
Organisme : CAMS Innovation Fund for Medical Sciences (CIFMS)
ID : 2022-I2M-1-021
Organisme : Science Fund for Creative Research Groups of the National Natural Science Foundation of China
ID : 82221004
Organisme : NCTIB Fund for R&D Platform for Cell and Gene Therapy
Organisme : Suzhou Municipal Key Laboratory
ID : SZS2023005
Informations de copyright
© 2024 The Authors. Advanced Science published by Wiley-VCH GmbH.
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