Image-activated pico-injection for single-cell analysis.

Algorithms Single-Cell Analysis / methods Cell Count
Label-free detection Pico-injection Real-time analysis Single-cell RNA sequencing

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
15 May 2024
Historique:
received: 30 09 2023
revised: 29 01 2024
accepted: 06 02 2024
medline: 18 3 2024
pubmed: 12 2 2024
entrez: 12 2 2024
Statut: ppublish

Résumé

The addition of reagents into preformed droplets is a crucial yet intricate task in droplet-based applications where sequential reactions is required. Pico-injection offers high throughput and robustness in accomplishing this task, but the existing pico-injection techniques work in an indiscriminate manner, making it difficult to target particular groups of droplets. Here we report image-activated pico-injection (imgPico) for label-free, on-demand reagent supplementation into droplets. The imgPico detects the droplets of interest by real-time image analysis and makes decisions for the downstream pico-injection operation. We studied the performance of different algorithms for the image analysis and optimized the experimental settings of the imgPico. In the validation experiment, the imgPico successfully injected fluorescent dyes into droplets encapsulating one, two, and three cells, respectively, as expected. We further demonstrated the utility of imgPico by targeting droplets encapsulating single cells in droplet-based single-cell RNA sequencing (scRNA-seq) using exceedingly high cell density, and the results showed that the imgPico effectively reduced the presence of doublets in the scRNA-seq data. With the merits of being label-free and versatile, the imgPico represents a technical advance with potential applications in single-cell analysis.

Identifiants

DOI: 10.1016/j.talanta.2024.125765 PMID: 38346358
pubmed: 38346358
pii: S0039-9140(24)00144-9
doi: 10.1016/j.talanta.2024.125765
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

125765

Informations de copyright

Copyright © 2024 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Zida Li reports a relationship with ThunderBio Innovation that includes: funding grants. Peng Zuo reports a relationship with ThunderBio Innovation that includes: employment. Ruyuan Song reports a relationship with ThunderBio Innovation that includes: employment. Xiaonan Xu reports a relationship with ThunderBio Innovation that includes: employment.

Auteurs

Zhantao Zhao (Z)

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

Heng Zhai (H)

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

Peng Zuo (P)

ThunderBio Innovation, Shenzhen, 518108, China.

Tao Wang (T)

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

Run Xie (R)

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

Mu Tian (M)

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

Ruyuan Song (R)

ThunderBio Innovation, Shenzhen, 518108, China.

Xiaonan Xu (X)

ThunderBio Innovation, Shenzhen, 518108, China.

Zida Li (Z)

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China. Electronic address: zidali@szu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Algorithmes › Image-activated pico-injection for single-cell...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Image-activated pico-injection for single-cell...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Numération cellulaire › Image-activated pico-injection for single-cell...