Isolation of Membrane Bound Mucins from Human Bronchial Epithelial Cells.

Human bronchial epithelial cells MUC1 MUC16 MUC4 Membrane-bound mucins

Journal

Methods in molecular biology (Clifton, N.J.)

ISSN: 1940-6029

Titre abrégé: Methods Mol Biol

Pays: United States

ID NLM: 9214969

Informations de publication

Date de publication:
2024

Historique:

medline: 13 2 2024

pubmed: 13 2 2024

entrez: 12 2 2024

Statut: ppublish

Résumé

Membrane-bound mucins constitute a large portion of the periciliary layer of lung epithelial surfaces, and thus play an important role in many aspects of innate defense. The biophysical and biochemical properties of the membrane-bound mucins have important implications for mucociliary clearance, viral penetration, and potential therapeutics delivered to the airway surface. Hence, isolating them and determining these properties is important in understanding airways disease and ultimately in developing treatments. Here, we describe a method using isopycnic centrifugation to enrich and isolate shed membrane-bound mucins from the washings of human bronchial epithelial cell cultures.

Identifiants

DOI: 10.1007/978-1-0716-3670-1_4 PMID: 38347399

pubmed: 38347399

doi: 10.1007/978-1-0716-3670-1_4

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

51-59

Informations de copyright

Références

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