Fabrication and Characterization of Mucin Nanoparticles for Drug Delivery Applications.
Antibiotic
DNA strand displacement
Glycoprotein
MUC5AC
Methacrylation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
13
2
2024
pubmed:
13
2
2024
entrez:
12
2
2024
Statut:
ppublish
Résumé
Mucin glycoproteins are ideal biomacromolecules for drug delivery applications since they naturally offer a plethora of different functional groups that can engage in specific and unspecific binding interactions with cargo molecules. However, to fabricate drug carrier objects from mucins, suitable stabilization mechanisms have to be implemented into the nanoparticle preparation procedure that allow for drug release profiles that match the requirements of the selected cargo molecule and its particular mode of action. Here, we describe two different methods to prepare crosslinked mucin nanoparticles that can release their cargo either on-demand or in a sustained manner. This method chapter includes a description of the preparation and characterization of mucin nanoparticles (stabilized either with synthetic DNA strands or with covalent crosslinks generated by free radical polymerization), as well as protocols to quantify the release of a model drug from those nanoparticles.
Identifiants
pubmed: 38347428
doi: 10.1007/978-1-0716-3670-1_33
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
383-394Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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