Characterizing the detection of inactivated Mycoplasma hyopneumoniae DNA in the respiratory tract of pigs.

DNA Mycoplasma hyopneumoniae PCR bacterial DNA diagnostics pigs

Journal

Veterinary research

ISSN: 1297-9716

Titre abrégé: Vet Res

Pays: England

ID NLM: 9309551

Informations de publication

Date de publication:
15 Feb 2024

Historique:

received: 17 10 2023

accepted: 20 11 2023

medline: 16 2 2024

pubmed: 16 2 2024

entrez: 15 2 2024

Statut: epublish

Résumé

A positive Mycoplasma hyopneumoniae PCR result in a clinical specimen may eventually represent the mere detection of non-viable bacteria, complicating the diagnostic interpretation. Thus, the objective of this study was to evaluate the PCR detection of non-viable M. hyopneumoniae and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivated M. hyopneumoniae and were sampled for up to 14 days. Mycoplasma hyopneumoniae was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viable M. hyopneumoniae DNA was rapidly sensed and cleared.

Identifiants

DOI: 10.1186/s13567-024-01273-2 PMID: 38360700

pubmed: 38360700

doi: 10.1186/s13567-024-01273-2

pii: 10.1186/s13567-024-01273-2

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

Informations de copyright

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