Identification of structural and regulatory cell-shape determinants in Haloferax volcanii.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
15 Feb 2024
15 Feb 2024
Historique:
received:
15
03
2023
accepted:
16
01
2024
medline:
16
2
2024
pubmed:
16
2
2024
entrez:
15
2
2024
Statut:
epublish
Résumé
Archaea play indispensable roles in global biogeochemical cycles, yet many crucial cellular processes, including cell-shape determination, are poorly understood. Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on growth conditions. Here, we used a combination of iterative proteomics, genetics, and live-cell imaging to identify mutants that only form rods or disks. We compared the proteomes of the mutants with wild-type cells across growth phases, thereby distinguishing between protein abundance changes specific to cell shape and those related to growth phases. The results identified a diverse set of proteins, including predicted transporters, transducers, signaling components, and transcriptional regulators, as important for cell-shape determination. Through phenotypic characterization of deletion strains, we established that rod-determining factor A (RdfA) and disk-determining factor A (DdfA) are required for the formation of rods and disks, respectively. We also identified structural proteins, including an actin homolog that plays a role in disk-shape morphogenesis, which we named volactin. Using live-cell imaging, we determined volactin's cellular localization and showed its dynamic polymerization and depolymerization. Our results provide insights into archaeal cell-shape determination, with possible implications for understanding the evolution of cell morphology regulation across domains.
Identifiants
pubmed: 38360755
doi: 10.1038/s41467-024-45196-0
pii: 10.1038/s41467-024-45196-0
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1414Subventions
Organisme : National Science Foundation (NSF)
ID : NSF-MBC2222076
Organisme : National Science Foundation (NSF)
ID : NSF-MBC2222076
Organisme : National Science Foundation (NSF)
ID : NSF-MBC2222076
Organisme : National Science Foundation (NSF)
ID : NSF-MBC2222076
Organisme : National Science Foundation (NSF)
ID : NSF-MBC2222076
Organisme : National Science Foundation (NSF)
ID : NSF-MBC2222076
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : T32 AI 141393
Organisme : Human Frontier Science Program (HFSP)
ID : RGY0074/2021
Informations de copyright
© 2024. The Author(s).
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