The Yucatan miniature swine as a model for post-inflammatory hyperpigmentation.
animal model
hyperpigmentation
melanin
pathophysiology
pigmentation
Journal
Pigment cell & melanoma research
ISSN: 1755-148X
Titre abrégé: Pigment Cell Melanoma Res
Pays: England
ID NLM: 101318927
Informations de publication
Date de publication:
16 Feb 2024
16 Feb 2024
Historique:
revised:
15
12
2023
received:
03
04
2023
accepted:
09
01
2024
medline:
16
2
2024
pubmed:
16
2
2024
entrez:
16
2
2024
Statut:
aheadofprint
Résumé
Post-inflammatory hyperpigmentation (PIH) is a hypermelanosis that often occurs secondary to skin irritation or injury, especially in darker skin tones, for which there is currently a lack of effective treatment options. Few preclinical models are available to study PIH. Here, we show that the Yucatan miniature pig consistently develops PIH after skin injuries. Skin wounds were produced on Yucatan pigs by needle punches, full-thickness excisions, or burns. Wound sites were monitored and photographed regularly. Tissue samples were collected after 24 weeks and processed for histology/immunohistochemistry. Skin pigmentation and histologic changes were quantified by computer-assisted image analyses. All injury methods resulted in hyperpigmentation. Melanin content at the histologic level was quantified in the larger (burn and excision) wounds, showing a significant increase compared to uninjured skin. Increased melanin was found for both epidermal and dermal regions. Dermal melanin deposits were primarily clustered around the papillary vasculature, and were associated not with melanocytes but with leukocytes. The Yucatan miniature pig model recapitulates key clinical and histologic features of PIH in humans, including skin hyperpigmentation at both gross and histologic levels, and persistence of dermal melanin subsequent to injury. This model could be used to further our understanding of the etiology of PIH, and for new therapy development.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Adini, I., Ghosh, K., Adini, A., Chi, Z.-L., Yoshimura, T., Benny, O., Connor, K. M., Rogers, M. S., Bazinet, L., Birsner, A. E., Bielenberg, D. R., & D'Amato, R. J. (2014). Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment. Journal of Clinical Investigation, 124(1), 425-436. https://doi.org/10.1172/JCI69404
Dai, N.-T., Chang, H.-I., Wang, Y.-W., Fu, K.-Y., Huang, T.-C., Huang, N.-C., Li, J.-K., Hsieh, P.-S., Dai, L.-G., Hsu, C.-K., & Maitz, P. K. (2018). Restoration of skin pigmentation after deep partial or full-thickness burn injury. Advanced Drug Delivery Reviews, 123, 155-164. https://doi.org/10.1016/j.addr.2017.10.010
Isedeh, P., Kohli, I., Al-Jamal, M., Agbai, O. N., Chaffins, M., Devpura, S., Mahan, M., Vanderover, G., Lim, H. W., Matsui, M. S., & Hamzavi, I. H. (2016). An in vivo model for postinflammatory hyperpigmentation: An analysis of histological, spectroscopic, colorimetric and clinical traits. British Journal of Dermatology, 174(4), 862-868. https://doi.org/10.1111/bjd.14184
Kim, J. Y., Dunham, D. M., Supp, D. M., Sen, C. K., & Powell, H. M. (2016). Novel burn device for rapid, reproducible burn wound generation. Burns, 42(2), 384-391. https://doi.org/10.1016/j.burns.2015.08.027
Maghfour, J., Olayinka, J., Hamzavi, I. H., & Mohammad, T. F. (2022). A focused review on the pathophysiology of post-inflammatory hyperpigmentation. Pigment Cell & Melanoma Research, 35(3), 320-327. https://doi.org/10.1111/pcmr.13038
Masu, S., & Seiji, M. (1983). Pigmentary incontinence in fixed drug eruptions. Histologic and electron microscopic findings. Journal of the American Academy of Dermatology, 8(4), 525-532. https://doi.org/10.1016/s0190-9622(83)70060-5
Nakano, S., Abe, Y., Nakajima, K., Sano, S., Yamamoto, O., Wakamatsu, K., Ito, S., Hayashi, M., & Suzuki, T. (2021). Establishment of a mouse model for post-inflammatory hyperpigmentation. Pigment Cell & Melanoma Research, 34(1), 101-110. https://doi.org/10.1111/pcmr.12911
Passeron, T., Nouveau, S., Duval, C., Cardot-Leccia, N., Piffaut, V., Bourreau, E., Queille-Roussel, C., & Bernerd, F. (2018). Development and validation of a reproducible model for studying post-inflammatory hyperpigmentation. Pigment Cell & Melanoma Research, 31(5), 649-652. https://doi.org/10.1111/pcmr.12692
Regazzetti, C., De Donatis, G. M., Ghorbel, H. H., Cardot-Leccia, N., Ambrosetti, D., Bahadoran, P., Chignon-Sicard, B., Lacour, J. P., Ballotti, R., Mahns, A., & Passeron, T. (2015). Endothelial cells promote pigmentation through endothelin receptor B activation. Journal of Investigative Dermatology, 135(12), 3096-3104. https://doi.org/10.1038/jid.2015.332
Rodrigues, M., Kosaric, N., Bonham, C. A., & Gurtner, G. C. (2019). Wound healing: A cellular perspective. Physiological Reviews, 99(1), 665-706. https://doi.org/10.1152/physrev.00067.2017
Ruifrok, A. C., & Johnston, D. A. (2001). Quantification of histochemical staining by color deconvolution. Analytical and Quantitative Cytology and Histology, 23(4), 291-299. https://www.ncbi.nlm.nih.gov/pubmed/11531144
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D. J., Hartenstein, V., Eliceiri, K., Tomancak, P., & Cardona, A. (2012). Fiji: An open-source platform for biological-image analysis. Nature Methods, 9(7), 676-682. https://doi.org/10.1038/nmeth.2019
Tam, J., Wang, Y., Farinelli, W. A., Jiménez-Lozano, J., Franco, W., Sakamoto, F. H., Cheung, E. J., Purschke, M., Doukas, A. G., & Anderson, R. R. (2013). Fractional skin harvesting: Autologous skin grafting without donor-site morbidity. Plastic and Reconstructive Surgery. Global Open, 1(6), e47. https://doi.org/10.1097/GOX.0b013e3182a85a36
Vellaichamy, G., Kohli, I., Zubair, R., Yin, C., Braunberger, T., Nahhas, A. F., Nicholson, C., Mohammad, T. F., Isedeh, P., Lyons, A. B., Nartker, N., Al-Jamal, M., Matsui, M., Karaman-Jurukovska, N., Zhou, L., Lim, H. W., Mi, Q.-S., & Hamzavi, I. H. (2022). An in vivo model of postinflammatory hyperpigmentation and erythema: Clinical, colorimetric and molecular characteristics. British Journal of Dermatology, 186(3), 508-519. https://doi.org/10.1111/bjd.20804