Biophysical characterization of microbial rhodopsins with DSE motif.

H+ pumping activity flash photolysis metagenomics microbial rhodopsins photocycle

Journal

Biophysics and physicobiology
ISSN: 2189-4779
Titre abrégé: Biophys Physicobiol
Pays: Japan
ID NLM: 101675089

Informations de publication

Date de publication:
21 Mar 2023
Historique:
received: 01 02 2023
accepted: 07 03 2023
medline: 16 2 2024
pubmed: 16 2 2024
entrez: 16 2 2024
Statut: epublish

Résumé

Microbial rhodopsins are photoreceptive transmembrane proteins that transport ions or regulate other intracellular biological processes. Recent genomic and metagenomic analyses found many microbial rhodopsins with unique sequences distinct from known ones. Functional characterization of these new types of microbial rhodopsins is expected to expand our understanding of their physiological roles. Here, we found microbial rhodopsins having a DSE motif in the third transmembrane helix from members of the Actinobacteria. Although the expressed proteins exhibited blue-green light absorption, either no or extremely small outward H

Identifiants

DOI: 10.2142/biophysico.bppb-v20.s023 PMID: 38362324 PMC: PMC10865882
pubmed: 38362324
doi: 10.2142/biophysico.bppb-v20.s023
pii: JST.JSTAGE/biophysico/e201023
pmc: PMC10865882
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e201023

Informations de copyright

2023 THE BIOPHYSICAL SOCIETY OF JAPAN.

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

Auteurs

María Del Carmen Marín (MDC)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba 277-8581, Japan.

Alexander L Jaffe (AL)

Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA.
Department of Earth System Science, Stanford University, Stanford, CA 94305-4216, USA.

Patrick T West (PT)

Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA.

Masae Konno (M)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba 277-8581, Japan.
PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.

Jillian F Banfield (JF)

Innovative Genomics Institute, University of California, Berkeley, CA 94720-2151, USA.
Department of Earth and Planetary Science, University of California, Berkeley, CA 94720-4767, USA.
Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720-3114, USA.

Keiichi Inoue (K)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba 277-8581, Japan.

Classifications MeSH