Understanding and manipulating extracellular behaviors of Wnt ligands.
Diffusion
Fluorescence correlation spectroscopy (FCS)
Fluorescence decay after photoconversion (FDAP)
Fluorescence recovery after photobleaching (FRAP)
Morphogen
Nanobody
Protein binder
Single-chain variable fragment (scFv)
Synthetic biology
Wnt
Journal
In vitro cellular & developmental biology. Animal
ISSN: 1543-706X
Titre abrégé: In Vitro Cell Dev Biol Anim
Pays: Germany
ID NLM: 9418515
Informations de publication
Date de publication:
20 Feb 2024
20 Feb 2024
Historique:
received:
13
11
2023
accepted:
17
01
2024
medline:
21
2
2024
pubmed:
21
2
2024
entrez:
21
2
2024
Statut:
aheadofprint
Résumé
Wnt, a family of secreted signaling proteins, serves diverse functions in embryogenesis, organogenesis, cancer, and stem cell functions. In the context of development, Wnt has been considered a representative morphogen, forming concentration gradients to give positional information to cells or tissues. However, although gradients are often illustrated in schemata, the reality of concentration gradients, or in other words, actual spatial distribution of Wnt ligands, and their behaviors in the extracellular space still remain poorly known. To understand extracellular behavior of Wnt ligands, quantitative analyses such as fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are highly informative because Wnt dispersal involves physical and biochemical processes, such as diffusion and binding to or dissociation from cell surface molecules, including heparan sulfate proteoglycans (HSPGs). Here, I briefly discuss representative methods to quantify morphogen dynamics. In addition, I discuss molecular manipulations of morphogens, mainly focusing on use of protein binders, and synthetic biology of morphogens as indicators of current and future directions in this field.
Identifiants
pubmed: 38379096
doi: 10.1007/s11626-024-00856-9
pii: 10.1007/s11626-024-00856-9
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 22K19335
Organisme : Japan Society for the Promotion of Science
ID : 22H02637
Organisme : Japan Society for the Promotion of Science
ID : 23H04930
Organisme : Precursory Research for Embryonic Science and Technology
ID : JPMJPR194B
Informations de copyright
© 2024. The Society for In Vitro Biology.
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