The core exosome proteome of Trichomonas vaginalis.

Core proteome Exosome Extracellular vesicles Trichomonas vaginalis

Journal

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
ISSN: 1995-9133
Titre abrégé: J Microbiol Immunol Infect
Pays: England
ID NLM: 100956211

Informations de publication

Date de publication:
16 Feb 2024
Historique:
received: 31 07 2023
revised: 15 01 2024
accepted: 13 02 2024
medline: 22 2 2024
pubmed: 22 2 2024
entrez: 21 2 2024
Statut: aheadofprint

Résumé

Trichomonas vaginalis is parasitic protozoan that causes human urogenital infections. Accumulated reports indicated that exosomes released by this parasite play a crucial role in transmitting information and substances between cells during host-parasite interactions. Current knowledge on the protein contents in T. vaginalis exosome is mainly generated from three previous studies that used different T. vaginalis isolates as an experimental model. Whether T. vaginalis exosomes comprise a common set of proteins (core exosome proteome) is still unclear. To explore the core exosome proteome in T. vaginalis, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the contents of sucrose ultracentrifugation-enriched exosome and supernatant fractions isolated from six isolates. Transmission electron microscopy (TEM) confirmed the presence of exosomes in the enriched fraction. Proteomic analysis identified a total of 1870 proteins from exosomal extracts. There were 1207 exosomal-specific proteins after excluding 436 'non-core exosomal proteins'. Among these, 72 common exosomal-specific proteins were expressed in all six isolates. Compared with three published T. vaginalis exosome proteome datasets, we identified 16 core exosomal-specific proteins. These core exosomal-specific proteins included tetraspanin (TvTSP1), the classical exosome marker, and proteins mainly involved in catalytic activity and binding such as ribosomal proteins, ras-associated binding (Rab) proteins, and heterotrimeric G proteins. Our study highlighted the importance of using supernatant fraction from exosomal extract as a control to eliminate 'non-core exosomal proteins'. We compiled a reference core exosome proteome of T. vaginalis, which is essential for developing a fundamental understanding of exosome-mediated cell communication and host-parasite interaction.

Sections du résumé

BACKGROUND BACKGROUND
Trichomonas vaginalis is parasitic protozoan that causes human urogenital infections. Accumulated reports indicated that exosomes released by this parasite play a crucial role in transmitting information and substances between cells during host-parasite interactions. Current knowledge on the protein contents in T. vaginalis exosome is mainly generated from three previous studies that used different T. vaginalis isolates as an experimental model. Whether T. vaginalis exosomes comprise a common set of proteins (core exosome proteome) is still unclear.
METHODS METHODS
To explore the core exosome proteome in T. vaginalis, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the contents of sucrose ultracentrifugation-enriched exosome and supernatant fractions isolated from six isolates.
RESULTS RESULTS
Transmission electron microscopy (TEM) confirmed the presence of exosomes in the enriched fraction. Proteomic analysis identified a total of 1870 proteins from exosomal extracts. There were 1207 exosomal-specific proteins after excluding 436 'non-core exosomal proteins'. Among these, 72 common exosomal-specific proteins were expressed in all six isolates. Compared with three published T. vaginalis exosome proteome datasets, we identified 16 core exosomal-specific proteins. These core exosomal-specific proteins included tetraspanin (TvTSP1), the classical exosome marker, and proteins mainly involved in catalytic activity and binding such as ribosomal proteins, ras-associated binding (Rab) proteins, and heterotrimeric G proteins.
CONCLUSIONS CONCLUSIONS
Our study highlighted the importance of using supernatant fraction from exosomal extract as a control to eliminate 'non-core exosomal proteins'. We compiled a reference core exosome proteome of T. vaginalis, which is essential for developing a fundamental understanding of exosome-mediated cell communication and host-parasite interaction.

Identifiants

DOI: 10.1016/j.jmii.2024.02.003 PMID: 38383245
pubmed: 38383245
pii: S1684-1182(24)00040-9
doi: 10.1016/j.jmii.2024.02.003
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest All authors declare that they have no competing interests.

Auteurs

Seow-Chin Ong (SC)

Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan. Electronic address: seowchin.ong@gmail.com.

Hong-Wei Luo (HW)

Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan. Electronic address: maple890817@gmail.com.

Wei-Hung Cheng (WH)

Department of Parasitology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan. Electronic address: whcheng@gs.ncku.edu.tw.

Fu-Man Ku (FM)

Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan. Electronic address: mann731025@mail.cgu.edu.tw.

Chih-Yu Tsai (CY)

Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan. Electronic address: winnie255137@gmail.com.

Po-Jung Huang (PJ)

Department of Biomedical Sciences, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan; Genomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan. Electronic address: pjhuang@gap.cgu.edu.tw.

Chi-Ching Lee (CC)

Genomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Guishan District, Taoyuan City, Taiwan. Electronic address: chichinglee@cgu.edu.tw.

Yuan-Ming Yeh (YM)

Genomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan. Electronic address: ymyeh@cgmh.org.tw.

Rose Lin (R)

Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan. Electronic address: lavender@mail.cgu.edu.tw.

Cheng-Hsun Chiu (CH)

Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Linkou, Taiwan. Electronic address: chchiu@adm.cgmh.org.tw.

Petrus Tang (P)

Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Linkou, Taiwan. Electronic address: petang@mail.cgu.edu.tw.

Classifications MeSH