Multi-molecular hyperspectral PRM-SRS microscopy.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 Feb 2024
21 Feb 2024
Historique:
received:
13
06
2023
accepted:
26
01
2024
medline:
22
2
2024
pubmed:
22
2
2024
entrez:
21
2
2024
Statut:
epublish
Résumé
Lipids play crucial roles in many biological processes. Mapping spatial distributions and examining the metabolic dynamics of different lipid subtypes in cells and tissues are critical to better understanding their roles in aging and diseases. Commonly used imaging methods (such as mass spectrometry-based, fluorescence labeling, conventional optical imaging) can disrupt the native environment of cells/tissues, have limited spatial or spectral resolution, or cannot distinguish different lipid subtypes. Here we present a hyperspectral imaging platform that integrates a Penalized Reference Matching algorithm with Stimulated Raman Scattering (PRM-SRS) microscopy. Using this platform, we visualize and identify high density lipoprotein particles in human kidney, a high cholesterol to phosphatidylethanolamine ratio inside granule cells of mouse hippocampus, and subcellular distributions of sphingosine and cardiolipin in human brain. Our PRM-SRS displays unique advantages of enhanced chemical specificity, subcellular resolution, and fast data processing in distinguishing lipid subtypes in different organs and species.
Identifiants
pubmed: 38383552
doi: 10.1038/s41467-024-45576-6
pii: 10.1038/s41467-024-45576-6
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1599Subventions
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : R01GM149976
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : U01AI167892
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : 5R01NS111039
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : R21NS125395
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : U54DK134301
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : U54HL165443
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : U54CA132378
Organisme : Hellman Foundation
ID : Fellow Award
Organisme : UC | University of California, San Diego (UC San Diego)
ID : Startup funds
Informations de copyright
© 2024. The Author(s).
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