[Study of centrifuge conditions for preparing rabbit leukocyte-poor platelet-rich plasma by single centrifugation].

To explore the best centrifuge condition for preparing rabbit leukocyte-poor platelet-rich plasma (LP-PRP) by using single centrifugation method. Sixteen healthy New Zealand rabbits, aged 3-4 months, were utilized in the investigation. A total of 15 mL anticoagulated blood was extracted from the central ear artery of each rabbit, with a repeat of the blood collection procedure after 1 and 2 months. The obtained blood specimens were individually subjected to centrifugation at a radius of 16.7 cm and speeds of 1 200, 1 300, 1 400, and 1 500 r/min (equivalent to centrifugal forces of 269× The volume of LP-PRP obtained under all centrifugation conditions ranged from 1.8 to 7.6 mL. At a consistent centrifugal speed, an extension of centrifugation time leaded to a significant increase in the volume of LP-PRP, accompanied by a declining trend in the platelet enrichment coefficient of LP-PRP. When centrifuged for 2 minutes, the volume of LP-PRP at speeds of 1 200 and 1 300 r/min was less than 2.0 mL, while the volume of LP-PRP obtained under other conditions was more than 2.0 mL. When centrifuged for 4 and 5 minutes, the volume of LP-PRP obtained at each speed was more than 4 mL. LP-PRP with a platelet enrichment coefficient more than 2.0 could be prepared by centrifuging at 1 200 r/min for each time group and 1 300 r/min for 2 and 3 minutes, and the highest LP-PRP platelet enrichment coefficient could be obtained by centrifugation for 2 minutes at a speed of 1 200 r/min. The platelet recovery rates of LP-PRP obtained by centrifugation at 1 200 r/min for 4 and 5 minutes, as well as centrifugation at 1 400 r/min for 5 minutes, were both greater than 60%. There was no significant difference between the groups when centrifuged at 1 200 r/min for 4 and 5 minutes ( In the process of preparing rabbit LP-PRP using a single centrifugation method, collecting 15 mL of blood and centrifuging at a radius of 16.7 cm and speed of 1 200 r/min for 4 minutes can prepare LP-PRP with a volume exceeding 2.0 mL, platelet enrichment coefficient exceeding 2.0, and platelet recovery rate exceeding 60%. This centrifugal condition can achieve the optimal LP-PRP action parameters in the shortest possible time. 探究使用一次离心法制备兔贫白细胞富血小板血浆（leukocyte-poor platelet-rich plasma，LP-PRP）的最佳离心条件。. 取16只3～4月龄健康新西兰兔，于兔耳背中央动脉采集15 mL抗凝血，分别于1、2个月后重复取血步骤。将所取血液标本于离心半径16.7 cm分别以转速1 200、1 300、1 400、1 500 r/min（对应离心力分别为269× 所有离心条件下获得的LP-PRP体积范围为1.8～7.6 mL。相同离心转速时，随离心时间延长，LP-PRP体积显著增大，同时LP-PRP血小板富集系数呈降低趋势。离心2 min时，1 200、1 300 r/min转速下LP-PRP体积<2.0 mL，其余条件下获得的LP-PRP体积均≥2.0 mL；离心4、5 min时，各转速下获得的LP-PRP体积均≥4.0 mL。1 200 r/min转速下各离心时间组和1 300 r/min转速下离心2、3 min组LP-PRP血小板富集系数均>2.0，其中1 200 r/min转速下离心2 min能够获得最高的LP-PRP血小板富集系数。1 200 r/min转速下离心4、5 min与1 400 r/min转速下离心5 min获得的LP-PRP血小板回收率均>60%，1 200 r/min转速下离心4、5 min组间比较差异无统计学意义（ 采用一次离心法制备兔LP-PRP时，采集15 mL血液以离心半径16.7 cm、1 200 r/min转速下离心4 min即可制备体积>2.0 mL、血小板富集系数>2.0且血小板回收率>60%的LP-PRP，在最短时间达到LP-PRP的最佳作用参数。.