Measurement of short-chain fatty acids in human plasma by means of fast gas chromatography-mass spectrometry.

Fast GC–MS Gas chromatography Human plasma SCFAs Short-chain fatty acids

Journal

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554

Informations de publication

Date de publication:
16 Feb 2024
Historique:
received: 31 07 2023
revised: 10 01 2024
accepted: 07 02 2024
medline: 23 2 2024
pubmed: 23 2 2024
entrez: 22 2 2024
Statut: aheadofprint

Résumé

A rapid and practicable analytical method for the measurement of short-chain fatty acids (SCFAs) in human plasma was developed. The extraction procedure involved the use of acidified water and methyl tert-butyl ether (MTBE), while the separation and detection of SCFAs, including acetic, propionic, and butyric acids was carried out by using gas chromatography-mass spectrometry (GC-MS) technique. The novelty of the research involves reducing the analysis time (less than 7 min) by using the novel fast GC-MS method. A narrow-bore GC capillary column of dimensions 30 m × 0.25 mm ID × 0.25 μm d

Identifiants

DOI: 10.1016/j.jchromb.2024.124044 PMID: 38387342
pubmed: 38387342
pii: S1570-0232(24)00052-7
doi: 10.1016/j.jchromb.2024.124044
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

124044

Informations de copyright

Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Giuseppe Micalizzi (G)

Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc 98168, Messina, Italy.

Chiara Buzzanca (C)

Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc 98168, Messina, Italy.

Valentina Chiaia (V)

Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc 98168, Messina, Italy.

Monica Mondello (M)

Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc 98168, Messina, Italy.

Francesco Cacciola (F)

Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria 98125, Messina, Italy. Electronic address: cacciolaf@unime.it.

Daniela Caccamo (D)

Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria 98125, Messina, Italy.

Luigi Mondello (L)

Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc 98168, Messina, Italy; Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci Snc 98168, Messina, Italy.

Classifications MeSH