Platform development for high-throughput optimization of perfusion processes: Part I: Implementation of cell bleeds in microwell plates.
deepwell plate
high-throughput
microwell plate
perfusion
process development
small-scale
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
23 Feb 2024
23 Feb 2024
Historique:
revised:
12
01
2024
received:
25
08
2023
accepted:
12
02
2024
medline:
23
2
2024
pubmed:
23
2
2024
entrez:
23
2
2024
Statut:
aheadofprint
Résumé
The promise of continuous processing to increase yields and improve product quality of biopharmaceuticals while decreasing the manufacturing footprint is transformative. Developing and optimizing perfusion operations requires screening various parameters, which is expensive and time-consuming when using benchtop bioreactors. Scale-down models (SDMs) are the most feasible option for high-throughput data generation and condition screening. However, new SDMs mimicking perfusion are required, enabling experiments to be run in parallel. In this study, a method using microwell plates (MWP) operating in semi-perfusion mode with an implemented cell bleed step is presented. A CHO cell line was cultivated in a 24-well MWP (V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : AstraZeneca
Organisme : Engineering and Physical Sciences Research Council
Informations de copyright
© 2024 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.
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