New combined absorption/

1H NMR analysis Biodegradation Bis(2-hydroxyethyl) terephthalic acid Bulk absorbance measurement Ideonella sakaiensis MHETase Mono(2-hydroxyethyl) terephthalic acid PETase Terephthalic acid

Journal

Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769

Informations de publication

Date de publication:
23 Feb 2024
Historique:
received: 11 11 2022
accepted: 10 02 2024
medline: 23 2 2024
pubmed: 23 2 2024
entrez: 23 2 2024
Statut: aheadofprint

Résumé

Poly(ethylene terephthalate) (PET) is a very valuable and beneficial material for industrial purposes, with various different applications. Due to the high annual production volume of over 50 million tons worldwide and the indiscriminate disposal by consumers, the polymers accumulate in the environment, causing negative effects on various ecosystems. Biodegradation via suitable enzymes represents a promising approach to combat the plastic waste issue so validated methods are required to measure the efficiency and efficacy of these enzymes. PETase and MHETase from Ideonella sakaiensis are suitable enzymes needed in combination to completely degrade PET into its environmentally friendly monomers. In this project, we compare and combine a previously described bulk absorbance measurement method with a newly established

Identifiants

pubmed: 38393574
doi: 10.1007/s11356-024-32481-0
pii: 10.1007/s11356-024-32481-0
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s).

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Auteurs

David Kornberger (D)

Faculty Medical and Life Sciences, Institute of Applied Biology, Molecular Biology Lab, Furtwangen University, Jakob-Kienzle-Str. 17, 78054, Villingen-Schwenningen, Germany.

Tanja Paatsch (T)

Faculty Medical and Life Sciences, Institute of Applied Biology, Molecular Biology Lab, Furtwangen University, Jakob-Kienzle-Str. 17, 78054, Villingen-Schwenningen, Germany.

Magnus Schmidt (M)

Faculty Medical and Life Sciences, Institute of Precision Medicine, Organic and Bioorganic Chemistry Labs, Furtwangen University, Jakob-Kienzle-Str. 17, 78054, Villingen-Schwenningen, Germany.

Ulrike Salat (U)

Faculty Medical and Life Sciences, Institute of Applied Biology, Molecular Biology Lab, Furtwangen University, Jakob-Kienzle-Str. 17, 78054, Villingen-Schwenningen, Germany. ulrike.salat@hs-furtwangen.de.

Classifications MeSH