Thylakoid membrane stacking controls electron transport mode during the dark-to-light transition by adjusting the distances between PSI and PSII.


Journal

Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677

Informations de publication

Date de publication:
23 Feb 2024
Historique:
received: 27 04 2023
accepted: 23 01 2024
medline: 24 2 2024
pubmed: 24 2 2024
entrez: 23 2 2024
Statut: aheadofprint

Résumé

The balance between linear electron transport (LET) and cyclic electron transport (CET) plays an essential role in plant adaptation and protection against photo-induced damage. This balance is largely maintained by phosphorylation-driven alterations in the PSII-LHCII assembly and thylakoid membrane stacking. During the dark-to-light transition, plants shift this balance from CET, which prevails to prevent overreduction of the electron transport chain and consequent photo-induced damage, towards LET, which enables efficient CO

Identifiants

pubmed: 38396112
doi: 10.1038/s41477-024-01628-9
pii: 10.1038/s41477-024-01628-9
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : United States-Israel Binational Science Foundation (BSF)
ID : 2019695
Organisme : United States-Israel Binational Science Foundation (BSF)
ID : 2019695
Organisme : United States-Israel Binational Science Foundation (BSF)
ID : 2015839
Organisme : Israel Science Foundation (ISF)
ID : 1082/17
Organisme : Israel Science Foundation (ISF)
ID : 1377/18
Organisme : National Science Foundation (NSF)
ID : 1616982

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature Limited.

Références

Bussi, Y. et al. Fundamental helical geometry consolidates the plant photosynthetic membrane. Proc. Natl Acad. Sci. USA 116, 22366–22375 (2019).
pubmed: 31611387 pmcid: 6825288 doi: 10.1073/pnas.1905994116
Shimoni, E., Rav-hon, O., Ohad, I., Brumfeld, V. & Reich, Z. Three-dimensional organization of higher-plant chloroplast thylakoid membranes revealed by electron tomography. Plant Cell 17, 2580–2586 (2005).
pubmed: 16055630 pmcid: 1197436 doi: 10.1105/tpc.105.035030
Nevo, R., Charuvi, D., Tsabari, O. & Reich, Z. Composition, architecture and dynamics of the photosynthetic apparatus in higher plants. Plant J. 70, 157–176 (2012).
pubmed: 22449050 doi: 10.1111/j.1365-313X.2011.04876.x
Daum, B., Nicastro, D., Austin, J., Richard McIntosh, J. & Kühlbrandt, W. Arrangement of photosystem II and ATP synthase in chloroplast membranes of spinach and pea. Plant Cell 22, 1299–1312 (2010).
pubmed: 20388855 pmcid: 2879734 doi: 10.1105/tpc.109.071431
Kirchhoff, H. et al. Structural and functional self-organization of photosystem II in grana thylakoids. Biochim. Biophys. Acta Bioenerg. 1767, 1180–1188 (2007).
doi: 10.1016/j.bbabio.2007.05.009
Rantala, M., Rantala, S. & Aro, E. M. Composition, phosphorylation and dynamic organization of photosynthetic protein complexes in plant thylakoid membrane. Photochem. Photobiol. Sci. 19, 604–619 (2020).
pubmed: 32297616 doi: 10.1039/d0pp00025f
Tikkanen, M., Nurmi, M., Kangasjärvi, S. & Aro, E. M. Core protein phosphorylation facilitates the repair of photodamaged photosystem II at high light. Biochim. Biophys. Acta Bioenerg. 1777, 1432–1437 (2008).
doi: 10.1016/j.bbabio.2008.08.004
Puthiyaveetil, S. et al. Compartmentalization of the protein repair machinery in photosynthetic membranes. Proc. Natl Acad. Sci. USA 111, 15839–15844 (2014).
pubmed: 25331882 pmcid: 4226077 doi: 10.1073/pnas.1413739111
Koochak, H., Puthiyaveetil, S., Mullendore, D. L., Li, M. & Kirchhoff, H. The structural and functional domains of plant thylakoid membranes. Plant J. 97, 412–429 (2019).
pubmed: 30312499 doi: 10.1111/tpj.14127
Anderson, J. M. The grana margins of plant thylakoid membranes. Physiol. Plant. 76, 243–248 (1989).
doi: 10.1111/j.1399-3054.1989.tb05640.x
Albertsson, P. Å. A quantitative model of the domain structure of the photosynthetic membrane. Trends Plant Sci. 6, 349–354 (2001).
pubmed: 11495787 doi: 10.1016/S1360-1385(01)02021-0
Armond, P. A., Staehelin, L. A. & Arntzen, C. J. Spatial relationship of photosystem I, photosystem II, and the light-harvesting complex in chloroplast membranes. J. Cell Biol. 73, 400–418 (1977).
pubmed: 870501 doi: 10.1083/jcb.73.2.400
Dekker, J. P. & Boekema, E. J. Supramolecular organization of thylakoid membrane proteins in green plants. Biochim. Biophys. Acta Bioenerg. 1706, 12–39 (2005).
doi: 10.1016/j.bbabio.2004.09.009
Wietrzynski, W. et al. Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision. eLife 9, e53740 (2020).
pubmed: 32297859 pmcid: 7164959 doi: 10.7554/eLife.53740
Puthiyaveetil, S., Van Oort, B. & Kirchhoff, H. Surface charge dynamics in photosynthetic membranes and the structural consequences. Nat. Plants 3, 17020 (2017).
pubmed: 28263304 doi: 10.1038/nplants.2017.20
Barber, J. Influence of surface charges on thylakoid structure and function. Annu. Rev. Plant Physiol. 33, 261–295 (1982).
doi: 10.1146/annurev.pp.33.060182.001401
Anderson, J. M., Horton, P., Kim, E. H. & Chow, W. S. Towards elucidation of dynamic structural changes of plant thylakoid architecture. Phil. Trans. R. Soc. B 367, 3515–3524 (2012).
pubmed: 23148278 pmcid: 3497079 doi: 10.1098/rstb.2012.0373
Chow, W. S., Kim, E.-H., Horton, P. & Anderson, J. M. Granal stacking of thylakoid membranes in higher plant chloroplasts: the physicochemical forces at work and the functional consequences that ensue. Photochem. Photobiol. Sci. 4, 1081–1090 (2005).
pubmed: 16307126 doi: 10.1039/b507310n
Kirchhoff, H. Architectural switches in plant thylakoid membranes. Photosynth. Res. 116, 481–487 (2013).
pubmed: 23677426 doi: 10.1007/s11120-013-9843-0
Fridlyand, L. E., Backhausen, J. E., Holtgrefe, S., Kitzmann, C. & Scheibe, R. Quantitative evaluation of the rate of 3-phosphoglycerate reduction in chloroplasts. Plant Cell Physiol. 38, 1177–1186 (1997).
doi: 10.1093/oxfordjournals.pcp.a029104
Robinson, S. P. & Walker, D. A. The control of 3-phosphoglycerate reduction in isolated chloroplasts by the concentrations of ATP, ADP and 3-phosphoglycerate. Biochim. Biophys. Acta Bioenerg. 545, 528–536 (1979).
doi: 10.1016/0005-2728(79)90161-0
Horton, P. in Photosynthetic Mechanisms and the Environment (eds Barber, J. & Baker, N. R.) 135–187 (Elsevier, 1985).
Hepworth, C. et al. Dynamic thylakoid stacking and state transitions work synergistically to avoid acceptor-side limitation of photosystem I. Nat. Plants 7, 87–98 (2021).
pubmed: 33432159 doi: 10.1038/s41477-020-00828-3
Li, Z., Wakao, S., Fischer, B. B. & Niyogi, K. K. Sensing and responding to excess light. Annu. Rev. Plant Biol. 60, 239–260 (2009).
pubmed: 19575582 doi: 10.1146/annurev.arplant.58.032806.103844
Miyake, C. Molecular mechanism of oxidation of p700 and suppression of ROS production in photosystem I in response to electron-sink limitations in C
pubmed: 32168828 pmcid: 7139980 doi: 10.3390/antiox9030230
Murata, N., Takahashi, S., Nishiyama, Y. & Allakhverdiev, S. I. Photoinhibition of photosystem II under environmental stress. Biochim. Biophys. Acta Bioenerg. 1767, 414–421 (2007).
doi: 10.1016/j.bbabio.2006.11.019
Fristedt, R., Granath, P. & Vener, A. V. A protein phosphorylation threshold for functional stacking of plant photosynthetic membranes. PLoS ONE 5, e10963 (2010).
pubmed: 20532038 pmcid: 2881038 doi: 10.1371/journal.pone.0010963
Tikkanen, M. et al. Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants. Biochim. Biophys. Acta Bioenerg. 1777, 425–432 (2008).
doi: 10.1016/j.bbabio.2008.02.001
Wood, W. H. J. et al. Dynamic thylakoid stacking regulates the balance between linear and cyclic photosynthetic electron transfer. Nat. Plants 4, 116–127 (2018).
pubmed: 29379151 doi: 10.1038/s41477-017-0092-7
Chuartzman, S. G. et al. Thylakoid membrane remodeling during state transitions in Arabidopsis. Plant Cell 20, 1029–1039 (2008).
pubmed: 18398051 pmcid: 2390732 doi: 10.1105/tpc.107.055830
Johnson, G. N. Physiology of PSI cyclic electron transport in higher plants. Biochim. Biophys. Acta Bioenerg. 1807, 384–389 (2011).
doi: 10.1016/j.bbabio.2010.11.009
Joliot, P. & Joliot, A. Cyclic electron transfer in plant leaf. Proc. Natl Acad. Sci. USA 99, 10209–10214 (2002).
pubmed: 12119384 pmcid: 126649 doi: 10.1073/pnas.102306999
Slovacek, R. E., Crowther, D. & Hind, G. Relative activities of linear and cyclic electron flows during chloroplast CO
doi: 10.1016/0005-2728(80)90094-8
Hertle, A. P. et al. PGRL1 is the elusive ferredoxin-plastoquinone reductase in photosynthetic cyclic electron flow. Mol. Cell 49, 511–523 (2013).
pubmed: 23290914 doi: 10.1016/j.molcel.2012.11.030
Munekage, Y. et al. Cyclic electron flow around photosystem I is essential for photosynthesis. Nature 429, 579–582 (2004).
pubmed: 15175756 doi: 10.1038/nature02598
Suorsa, M. et al. PROTON GRADIENT REGULATION5 is essential for proper acclimation of Arabidopsis photosystem I to naturally and artificially fluctuating light conditions. Plant Cell 24, 2934–2948 (2012).
pubmed: 22822205 pmcid: 3426124 doi: 10.1105/tpc.112.097162
Tikkanen, M., Grieco, M., Kangasjärvi, S. & Aro, E. M. Thylakoid protein phosphorylation in higher plant chloroplasts optimizes electron transfer under fluctuating light. Plant Physiol. 152, 723–735 (2010).
pubmed: 19965965 pmcid: 2815896 doi: 10.1104/pp.109.150250
Telfer, A., Hodges, M., Millner, P. A. & Barber, J. The cation-dependence of the degree of protein phosphorylation-induced unstacking of pea thylakoids. Biochim. Biophys. Acta Bioenerg. 766, 554–562 (1984).
doi: 10.1016/0005-2728(84)90114-2
Höhner, R. et al. Plastocyanin is the long-range electron carrier between photosystem II and photosystem I in plants. Proc. Natl Acad. Sci. USA 117, 15354–15362 (2020).
pubmed: 32541018 pmcid: 7334583 doi: 10.1073/pnas.2005832117
Pribil, M., Pesaresi, P., Hertle, A., Barbato, R. & Leister, D. Role of plastid protein phosphatase TAP38 in LHCII dephosphorylation and thylakoid electron flow. PLoS Biol. 8, e1000288 (2010).
pubmed: 20126264 pmcid: 2811158 doi: 10.1371/journal.pbio.1000288
Kirchhoff, H. et al. Dynamic control of protein diffusion within the granal thylakoid lumen. Proc. Natl Acad. Sci. USA 108, 20248–20253 (2011).
pubmed: 22128333 pmcid: 3250138 doi: 10.1073/pnas.1104141109
Johnson, M. P. & Wientjes, E. The relevance of dynamic thylakoid organisation to photosynthetic regulation. Biochim. Biophys. Acta Bioenerg. 1861, 148039 (2020).
pubmed: 31228404 doi: 10.1016/j.bbabio.2019.06.011
Staehelin, L. A. in Photosynthesis III (eds Staehelin, L. A. & Arntzen, C. J.) 1–84 (Springer Berlin, 1986); https://doi.org/10.1007/978-3-642-70936-4_1
Pesaresi, P., Pribil, M., Wunder, T. & Leister, D. Dynamics of reversible protein phosphorylation in thylakoids of flowering plants: the roles of STN7, STN8 and TAP38. Biochim. Biophys. Acta Bioenerg. 1807, 887–896 (2011).
doi: 10.1016/j.bbabio.2010.08.002
Tikkanen, M. & Aro, E. M. Thylakoid protein phosphorylation in dynamic regulation of photosystem II in higher plants. Biochim. Biophys. Acta Bioenerg. 1817, 232–238 (2012).
doi: 10.1016/j.bbabio.2011.05.005
Longoni, P., Samol, I. & Goldschmidt-Clermont, M. The kinase STATE TRANSITION 8 phosphorylates light harvesting complex II and contributes to light acclimation in Arabidopsis thaliana. Front. Plant Sci. 10, 1156 (2019).
pubmed: 31608094 pmcid: 6761601 doi: 10.3389/fpls.2019.01156
Bellaflore, S., Barneche, F., Peltler, G. & Rochalx, J. D. State transitions and light adaptation require chloroplast thylakoid protein kinase STN7. Nature 433, 892–895 (2005).
doi: 10.1038/nature03286
Samol, I. et al. Identification of a photosystem II phosphatase involved in light acclimation in Arabidopsis. Plant Cell 24, 2596–2609 (2012).
pubmed: 22706287 pmcid: 3406908 doi: 10.1105/tpc.112.095703
Rochaix, J.-D. et al. Protein kinases and phosphatases involved in the acclimation of the photosynthetic apparatus to a changing light environment. Phil. Trans. R. Soc. B 367, 3466–3474 (2012).
pubmed: 23148273 pmcid: 3497069 doi: 10.1098/rstb.2012.0064
Shapiguzov, A. et al. The PPH1 phosphatase is specifically involved in LHCII dephosphorylation and state transitions in Arabidopsis. Proc. Natl Acad. Sci. USA 107, 4782–4787 (2010).
pubmed: 20176943 pmcid: 2842063 doi: 10.1073/pnas.0913810107
Charuvi, D., Nevo, R., Kaplan-Ashiri, I., Shimoni, E. & Reich, Z. Studying the supramolecular organization of photosynthetic membranes within freeze-fractured leaf tissues by cryo-scanning electron microscopy. J. Vis. Exp. https://doi.org/10.3791/54066 (2016).
Branton, D. Fracture faces of frozen membranes: 50th anniversary. Mol. Biol. Cell 27, 421–423 (2016).
pubmed: 26823391 pmcid: 4751593 doi: 10.1091/mbc.e15-05-0287
Staehelin, L. A. Reversible particle movements associated with unstacking and restacking of chloroplast membranes in vitro. J. Cell Biol. 71, 136–158 (1976).
pubmed: 988028 doi: 10.1083/jcb.71.1.136
Wollman, F. A., Olive, J., Bennoun, P. & Recouvreur, M. Organization of the photosystem II centers and their associated antennae in the thylakoid membranes: a comparative ultrastructural, biochemical, and biophysical study of Chlamydomonas wild type and mutants lacking in photosystem II reaction centers. J. Cell Biol. 87, 728–735 (1980).
pubmed: 7462323 doi: 10.1083/jcb.87.3.728
Staehelin, L. A. & van der Staay, G. W. M. in Oxygenic Photosynthesis: The Light Reactions (eds Ort, D. R. et al.) 11–30 (Springer Netherlands, 1996); https://doi.org/10.1007/0-306-48127-8_2
Armond, P. A. & Arntzen, C. J. Localization and characterization of photosystem II in grana and stroma lamellae. Plant Physiol. 59, 398–404 (1977).
pubmed: 16659861 pmcid: 542412 doi: 10.1104/pp.59.3.398
Hankamer, B., Barber, J. & Boekema, E. J. Structure and membrane organization of PSII in green plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 641–671 (1997).
pubmed: 15012277 doi: 10.1146/annurev.arplant.48.1.641
Fristedt, R. et al. Phosphorylation of photosystem II controls functional macroscopic folding of photosynthetic membranes in Arabidopsis. Plant Cell 21, 3950–3964 (2009).
pubmed: 20028840 pmcid: 2814517 doi: 10.1105/tpc.109.069435
Armbruster, U. et al. Arabidopsis CURVATURE THYLAKOID1 proteins modify thylakoid architecture by inducing membrane curvature. Plant Cell 25, 2661–2678 (2013).
pubmed: 23839788 pmcid: 3753390 doi: 10.1105/tpc.113.113118
Kirchhoff, H. Diffusion of molecules and macromolecules in thylakoid membranes. Biochim. Biophys. Acta Bioenerg. 1837, 495–502 (2014).
doi: 10.1016/j.bbabio.2013.11.003
Kirchhoff, H., Schöttler, M. A., Maurer, J. & Weis, E. Plastocyanin redox kinetics in spinach chloroplasts: evidence for disequilibrium in the high potential chain. Biochim. Biophys. Acta Bioenerg. 1659, 63–72 (2004).
doi: 10.1016/j.bbabio.2004.08.004
Wood, W. H. J. & Johnson, M. P. Modeling the role of LHCII–LHCII, PSII–LHCII, and PSI–LHCII interactions in state transitions. Biophys. J. 119, 287–299 (2020).
pubmed: 32621865 pmcid: 7376088 doi: 10.1016/j.bpj.2020.05.034
Trissl, H. W. & Wilhelm, C. Why do thylakoid membranes from higher plants form grana stacks? Trends Biochem. Sci. 18, 415–419 (1993).
pubmed: 8291084 doi: 10.1016/0968-0004(93)90136-B
Anderson, J. M. Insights into the consequences of grana stacking of thylakoid membranes in vascular plants: a personal perspective. Aust. J. Plant Physiol. 26, 625–639 (1999).
Pribil, M., Labs, M. & Leister, D. Structure and dynamics of thylakoids in land plants. J. Exp. Bot. 65, 1955–1972 (2014).
pubmed: 24622954 doi: 10.1093/jxb/eru090
Wood, W. H. J., Barnett, S. F. H., Flannery, S., Hunter, C. N. & Johnson, M. P. Dynamic thylakoid stacking is regulated by LHCII phosphorylation but not its interaction with PSI. Plant Physiol. 180, 2152–2166 (2019).
pmcid: 6670114 doi: 10.1104/pp.19.00503
Tsabari, O. et al. Differential effects of ambient or diminished CO
pubmed: 25619921 doi: 10.1111/tpj.12774
Barber, J. An explanation for the relationship between salt-induced thylakoid stacking and the chlorophyll fluorescence changes associated with changes in spillover of energy from photosystem II to photosystem I. FEBS Lett. 118, 1–10 (1980).
doi: 10.1016/0014-5793(80)81207-5
Briantais, J. M., Vernotte, C., Olive, J. & Wollman, F. A. Kinetics of cation-induced changes of photosystem II fluorescence and of lateral distribution of the two photosystems in the thylakoid membranes of pea chloroplasts. Biochim. Biophys. Acta Bioenerg. 766, 1–8 (1984).
doi: 10.1016/0005-2728(84)90210-X
Yokono, M., Takabayashi, A., Akimoto, S. & Tanaka, A. A megacomplex composed of both photosystem reaction centres in higher plants. Nat. Commun. 6, 6675 (2015).
pubmed: 25809225 doi: 10.1038/ncomms7675
Järvi, S., Suorsa, M., Paakkarinen, V. & Aro, E. M. Optimized native gel systems for separation of thylakoid protein complexes: novel super- and mega-complexes. Biochem. J. 439, 207–214 (2011).
pubmed: 21707535 doi: 10.1042/BJ20102155
Grieco, M., Suorsa, M., Jajoo, A., Tikkanen, M. & Aro, E. M. Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery—including both photosystems II and II. Biochim. Biophys. Acta Bioenerg. 1847, 607–619 (2015).
doi: 10.1016/j.bbabio.2015.03.004
Rantala, S. & Tikkanen, M. Phosphorylation-induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct 2, e00039 (2018).
pubmed: 31245706 pmcid: 6508491 doi: 10.1002/pld3.39
Rantala, M. et al. Chloroplast acetyltransferase GNAT2 is involved in the organization and dynamics of thylakoid structure. Plant Cell Physiol. https://doi.org/10.1093/pcp/pcac096 (2022).
Rozak, P. R., Seiser, R. M., Wacholtz, W. F. & Wise, R. R. Rapid, reversible alterations in spinach thylakoid appression upon changes in light intensity. Plant Cell Environ. 25, 421–429 (2002).
doi: 10.1046/j.0016-8025.2001.00823.x
Nevo, R. et al. in Lipids in Photosynthesis: Essential and Regulatory Functions (eds Wada, H. & Murata, N.) Dordrecht Springer-Verlag Vol. 30, 295–328 (2009).
Li, M. et al. Measuring the dynamic response of the thylakoid architecture in plant leaves by electron microscopy. Plant Direct 4, e00280 (2020).
pubmed: 33195966 pmcid: 7644818 doi: 10.1002/pld3.280
Allen, J. F. Protein phosphorylation in regulation of photosynthesis. Biochim. Biophys. Acta Bioenerg. 1098, 275–335 (1992).
doi: 10.1016/S0005-2728(09)91014-3
Anderson, J. M. Consequences of spatial separation of photosystem 1 and 2 in thylakoid membranes of higher plant chloroplasts. FEBS Lett. 124, 1–10 (1981).
doi: 10.1016/0014-5793(81)80041-5
Anderson, J. M. The significance of grana stacking in chlorophyll B containing chloroplasts. Photobiochem. Photobiophys. 3, 225–241 (1982).
Suorsa, M. et al. Light acclimation involves dynamic re-organization of the pigment–protein megacomplexes in non-appressed thylakoid domains. Plant J. 84, 360–373 (2015).
pubmed: 26332430 doi: 10.1111/tpj.13004
Bag, P. et al. Direct energy transfer from photosystem II to photosystem I confers winter sustainability in Scots pine. Nat. Commun. 11, 6388 (2020).
pubmed: 33319777 pmcid: 7738668 doi: 10.1038/s41467-020-20137-9
Kramer, D. M., Johnson, G., Kiirats, O. & Edwards, G. E. New fluorescence parameters for the determination of QA redox state and excitation energy fluxes. Biol. Chem. 79, 209–218 (2004).
Walther, P. & Müller, M. Double-layer coating for field-emission cryo-scanning electron microscopy—present state and applications. Scanning 19, 343–348 (1997).
pubmed: 9262018 doi: 10.1002/sca.4950190501
Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671–675 (2012).
pubmed: 22930834 pmcid: 5554542 doi: 10.1038/nmeth.2089
Berg, S. et al. Ilastik: interactive machine learning for (bio)image analysis. Nat. Methods 16, 1226–1232 (2019).
pubmed: 31570887 doi: 10.1038/s41592-019-0582-9
R Core Team. R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing (2020).
Wickham, H. et al. Welcome to the Tidyverse. J. Open Source Softw. 4, 1686 (2019).
doi: 10.21105/joss.01686
Wientjes, E., Van Amerongen, H. & Croce, R. Quantum yield of charge separation in photosystem II: functional effect of changes in the antenna size upon light acclimation. J. Phys. Chem. B 117, 11200–11208 (2013).
pubmed: 23534376 doi: 10.1021/jp401663w

Auteurs

Yuval Garty (Y)

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.

Yuval Bussi (Y)

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.

Smadar Levin-Zaidman (S)

Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.

Eyal Shimoni (E)

Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.

Helmut Kirchhoff (H)

Institute of Biological Chemistry, Washington State University, Pullman, WA, USA.

Dana Charuvi (D)

Institute of Plant Sciences, Agricultural Research Organization, Volcani Institute, Rishon LeZion, Israel.

Reinat Nevo (R)

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel. reinat.nevo@weizmann.ac.il.

Ziv Reich (Z)

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel. ziv.reich@weizmann.ac.il.

Classifications MeSH