Artificial Antigen-Presenting Cell Fabrication for Murine T Cell Expansion.
CD8+ T cell expansion
T cell activation
artificial antigen-presenting cells
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Feb 2024
Feb 2024
Historique:
medline:
24
2
2024
pubmed:
24
2
2024
entrez:
24
2
2024
Statut:
ppublish
Résumé
Antigen-presenting cells (APCs), such as dendritic cells and macrophages, have a unique ability to survey the body and present information to T cells via peptide-loaded major histocompatibility complexes (signal 1). This presentation, along with a co-stimulatory signal (signal 2), leads to activation and subsequent expansion of T cells. This process can be harnessed and utilized for therapeutic applications, but the use of patient-derived APCs can be complex and inefficient. Alternatively, artificial APCs (aAPCs) provide a simplified method to achieve T cell activation by presenting the two necessary stimulatory signals. This protocol describes the utilization of magnetic nanoparticles and stimulatory proteins to create aAPCs that can be employed for activating and expanding antigen-specific T cells for both basic and translational immunology and immunotherapy studies. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Protein and particle modification for aAPC fabrication Basic Protocol 2: aAPC validation by immunolabeling of conjugated protein Support Protocol 1: Quantification of aAPC stock concentration Basic Protocol 3: Determination of aAPC usage for murine CD8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e976Subventions
Organisme : NIH HHS
ID : P41EB028239
Pays : United States
Organisme : NIH HHS
ID : R33CA229042
Pays : United States
Informations de copyright
© 2024 Wiley Periodicals LLC.
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