Mesenchymal stem cells-derived extracellular vesicles for therapeutics of renal tuberculosis.

Anti-tuberculosis therapy Extracellular vesicles Mesenchymal stem cells Proteins Renal tuberculosis

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 Feb 2024
Historique:
received: 11 11 2023
accepted: 19 02 2024
medline: 25 2 2024
pubmed: 25 2 2024
entrez: 24 2 2024
Statut: epublish

Résumé

Extrapulmonary tuberculosis with a renal involvement can be a manifestation of a disseminated infection that requires therapeutic intervention, particularly with a decrease in efficacy of conventional regimens. In the present study, we investigated the therapeutic potency of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in the complex anti-tuberculosis treatment (ATT). A rabbit model of renal tuberculosis (rTB) was constructed by injecting of the standard strain Mycobacterium tuberculosis H37Rv into the cortical layer of the kidney parenchyma. Isolated rabbit MSC-EVs were intravenously administered once as an addition to standard ATT (isoniazid, pyrazinamide, and ethambutol). The therapeutic efficacy was assessed by analyzing changes of blood biochemical biomarkers and levels of anti- and pro-inflammatory cytokines as well as by renal computed tomography with subsequent histological and morphometric examination. The therapeutic effect of therapy with MSC-EVs was shown by ELISA method that confirmed a statistically significant increase of the anti-inflammatory and decrease of pro-inflammatory cytokines as compared to conventional treatment. In addition, there is a positive trend in increase of ALP level, animal weigh, and normalization of ADA activity that can indicate an improvement of kidney state. A significant reduction of the area of specific and interstitial inflammation indicated positive affect of MSC-EVs that suggests a shorter duration of ATT. The number of MSC-EVs proteins (as identified by mass-spectometry analysis) with anti-microbial, anti-inflammatory and immunoregulatory functions reduced the level of the inflammatory response and the severity of kidney damage (further proved by morphometric analysis). In conclusion, MSC-EVs can be a promising tool for the complex treatment of various infectious diseases, in particularly rTB.

Identifiants

pubmed: 38402260
doi: 10.1038/s41598-024-54992-z
pii: 10.1038/s41598-024-54992-z
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4495

Subventions

Organisme : Saint-Petersburg State Research Institute of Phthisiopulmonology within the framework of the State task of the Ministry of Health of the Russian Federation
ID : N 121112600145-2)
Organisme : ministry of Education (MoE) Govt of India through the SPARC (Scheme for Promotion of Academic and Research Collaboration)
ID : No SPARC/2019-2020/P2420/SL
Organisme : Government of India under Indo-Russia cooperation program
ID : Project No. INT/RUS/RFBR/380

Informations de copyright

© 2024. The Author(s).

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Auteurs

Natalia Yudintceva (N)

Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave., 4, Saint Petersburg, Russia, 194064. yudintceva@mail.ru.

Danila Bobkov (D)

Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave., 4, Saint Petersburg, Russia, 194064.

Maksim Sulatsky (M)

Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave., 4, Saint Petersburg, Russia, 194064.

Natalia Mikhailova (N)

Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave., 4, Saint Petersburg, Russia, 194064.

Elena Oganesyan (E)

Personalized Medicine Centre, Almazov National Medical Research Centre, Akkuratova Str. 2, Saint Petersburg, Russia, 197341.

Tatiana Vinogradova (T)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Alexandr Muraviov (A)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.
Private University St. Petersburg Medico-Social Institute, Kondratievskiy Ave., 72A, Saint Petersburg, Russia, 195271.

Anna Remezova (A)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Evdokia Bogdanova (E)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Irina Garapach (I)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Olga Maslak (O)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Dilyara Esmedlyaeva (D)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Marina Dyakova (M)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Petr Yablonskiy (P)

Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, Ligovsky Ave., 2-4, Saint Petersburg, Russia, 191036.

Rustam Ziganshin (R)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.

Sergey Kovalchuk (S)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.

Natalya Blum (N)

Kirov Military Medical Academy, Akademika Lebedeva Str., 6, Saint Petersburg, Russia, 194044.

Shirish H Sonawane (SH)

National Institute of Technology Warangal, Warangal, 506004, India.

Avinash Sonawane (A)

Indian Institute of Technology Indore, Indore, 453552, India.

Ankita Behl (A)

Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, 110067, India.
Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, 110067, India.

Maxim Shevtsov (M)

Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave., 4, Saint Petersburg, Russia, 194064. maxim.shevtsov@tum.de.
Department of Radiation Oncology, Central Institute for Translational Cancer Research (TranslaTUM), Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany. maxim.shevtsov@tum.de.
School of Medicine and Life Sciences, Far Eastern Federal University, Campus 10 Ajax Bay, Russky Island, Vladivostok, Russia, 690922. maxim.shevtsov@tum.de.

Classifications MeSH