Effects of blood sample storage time, temperature, anti-coagulants and blood stabiliser on lymphocyte phenotyping.

Lymphocyte phenotyping blood processing delays blood stabilisers specimen storage

Journal

Pathology
ISSN: 1465-3931
Titre abrégé: Pathology
Pays: England
ID NLM: 0175411

Informations de publication

Date de publication:
07 Feb 2024
Historique:
received: 15 01 2023
revised: 30 10 2023
accepted: 20 11 2023
medline: 26 2 2024
pubmed: 26 2 2024
entrez: 25 2 2024
Statut: aheadofprint

Résumé

Medical diagnostic laboratories have come under further scrutiny to ensure quality standards of their service and external quality assurance (EQA) programs involving multiple laboratories have been used to gauge this quality based on a consensus. However, because of the geographical distances within a country or internationally, cell surface marker expressions may change due to time delays and transport temperatures. Attention was given to this issue some decades ago and hence requires a re-evaluation in consideration of updated methods, reagents and instruments for flow cytometry and phenotyping. We have undertaken an extensive study to examine the effects of various conditions on blood storage akin to that experienced by patient samples as well as EQA programs, examining expression of lymphocyte surface markers, CD3, CD4, CD8, CD2, CD19, CD20, CD16/56 and HLA-DR. Assessment of lithium-heparin anticoagulated whole blood showed an increase in percentage of CD3

Identifiants

DOI: 10.1016/j.pathol.2023.11.011 PMID: 38403560
pubmed: 38403560
pii: S0031-3025(24)00052-7
doi: 10.1016/j.pathol.2023.11.011
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Yunyu Lao (Y)

Department of Immunopathology, SA Pathology at Women's and Children's Hospital, North Adelaide, SA, Australia; Adelaide Medical School, School of Biomedicine and the Robinson Research Institute, Faculty of Health Science, University of Adelaide, Adelaide, SA, Australia.

Alex Quach (A)

Department of Immunopathology, SA Pathology at Women's and Children's Hospital, North Adelaide, SA, Australia; Adelaide Medical School, School of Biomedicine and the Robinson Research Institute, Faculty of Health Science, University of Adelaide, Adelaide, SA, Australia.

Khalida Perveen (K)

Department of Immunopathology, SA Pathology at Women's and Children's Hospital, North Adelaide, SA, Australia; Adelaide Medical School, School of Biomedicine and the Robinson Research Institute, Faculty of Health Science, University of Adelaide, Adelaide, SA, Australia.

Charles Hii (C)

Department of Immunopathology, SA Pathology at Women's and Children's Hospital, North Adelaide, SA, Australia; Adelaide Medical School, School of Biomedicine and the Robinson Research Institute, Faculty of Health Science, University of Adelaide, Adelaide, SA, Australia.

Antonio Ferrante (A)

Department of Immunopathology, SA Pathology at Women's and Children's Hospital, North Adelaide, SA, Australia; Adelaide Medical School, School of Biomedicine and the Robinson Research Institute, Faculty of Health Science, University of Adelaide, Adelaide, SA, Australia; School of Biological Sciences, Faculty of Science, University of Adelaide, Adelaide, SA, Australia. Electronic address: antonio.ferrante@adelaide.edu.au.

Classifications MeSH